Artículo
Expression of DNA methyltransferase genes in four-cell bovine embryos cultured in the presence of oviductal fluid
Barrera, Antonio Daniel
; Garcia, Elina Vanesa
; Sinowatz, F.; Palma, Gustavo Adolfo
; Jiménez Díaz, María Atilana; Miceli, Dora Cristina
Fecha de publicación:
08/2013
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Anatomia Histologia Embryologia-Journal Of Veterinary Medicine Series C
ISSN:
0340-2096
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The possible epigenomic effect of oviductal fluid on expression of DNA methyltransferase (DNMT) genes was examined in early bovine embryos (4-cell stage). Real-time quantitative PCR was performed to determine the relative expression of DNMT1, DNMT3a and DNMT3b transcripts in embryos cultured in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviductal fluid. These results may help reveal the role of oviductal factors in the regulation of DNMT expression. © 2012 Blackwell Verlag GmbH.
Palabras clave:
Oviduct
,
Embryos
,
Bovine
,
Methyl Transferasas
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Identificadores
Colecciones
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Barrera, Antonio Daniel; Garcia, Elina Vanesa; Sinowatz, F.; Palma, Gustavo Adolfo; Jiménez Díaz, María Atilana; et al.; Expression of DNA methyltransferase genes in four-cell bovine embryos cultured in the presence of oviductal fluid; Wiley Blackwell Publishing, Inc; Anatomia Histologia Embryologia-Journal Of Veterinary Medicine Series C; 42; 4; 8-2013; 312-315
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