Artículo
ROS enhancement by silicon nanoparticles in X-ray irradiated aqueous suspensions and in glioma C6 cells
David Gara, Pedro Maximiliano; Garabano, Natalia Ines
; Llansola Portolés, Manuel Jose
; Moreno, Mario Sergio Jesus
; Dodat, Diego; Casas, Oscar R.; Gonzalez, Monica Cristina
; Kotler, Monica Lidia
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Fecha de publicación:
04/2012
Editorial:
Springer
Revista:
Journal of Nanoparticle Research
ISSN:
1388-0764
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The capability of silicon nanoparticles to increase the yield of reactive species upon 4 MeV X-ray irradiation of aqueous suspensions and C6 glioma cell cultures was investigated. ROS generation was detected and quantified using several specific probes. The particles were characterized by FTIR, XPS, TEM, DLS, luminescence, and adsorption spectroscopy before and after irradiation to evaluate the effect of high energy radiation on their structure. The total concentration of O 2 ·-/HO 2 ·, HO ·, and H 2O 2 generated upon 4-MeV X-ray irradiation of 6.4 μM silicon nanoparticle aqueous suspensions were on the order of 10 μM per Gy, ten times higher than that obtained in similar experiments but in the absence of particles. Cytotoxic 1O 2 was generated only in irradiation experiments containing the particles. The particle surface became oxidized to SiO 2 and the luminescence yield reduced with the irradiation dose. Changes in the surface morphology did not affect, within the experimental error, the yields ofROSgenerated per Gy. X-ray irradiation of glioma C6 cell cultures with incorporated silicon nanoparticles showed a marked production of ROS proportional to the radiation dose received. In the absence of nanoparticles, the cells showed no irradiation- enhanced ROS generation. The obtained results indicate that silicon nanoparticles of <5 nm size have the potential to be used as radiosensitizers for improving the outcomes of cancer radiotherapy. Their capability of producing 1O 2 upon X-ray irradiation opens novel approaches in the design of therapy strategies. © Springer Science+Business Media B.V. 2012.
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Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
David Gara, Pedro Maximiliano; Garabano, Natalia Ines; Llansola Portolés, Manuel Jose; Moreno, Mario Sergio Jesus; Dodat, Diego; et al.; ROS enhancement by silicon nanoparticles in X-ray irradiated aqueous suspensions and in glioma C6 cells; Springer; Journal of Nanoparticle Research; 14; 3; 4-2012; 741-754
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