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dc.contributor.author
Regiart, Daniel Matias Gaston  
dc.contributor.author
Pereira, Sirley Vanesa  
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Bertolino, Franco Adrián  
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Garcia, Carlos D.  
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Raba, Julio  
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Aranda, Pedro Rodolfo  
dc.date.available
2018-09-21T21:48:59Z  
dc.date.issued
2016-03  
dc.identifier.citation
Regiart, Daniel Matias Gaston; Pereira, Sirley Vanesa; Bertolino, Franco Adrián; Garcia, Carlos D.; Raba, Julio; et al.; An electrochemical immunosensor for anti-T. cruzi IgM antibodies, a biomarker for congenital Chagas disease, using a screen-printed electrode modified with gold nanoparticles and functionalized with shed acute phase antigen; Springer Wien; Mikrochimica Acta; 183; 3; 3-2016; 1203-1210  
dc.identifier.issn
0026-3672  
dc.identifier.uri
http://hdl.handle.net/11336/60679  
dc.description.abstract
The authors describe a sandwich immunosensor for anti-IgM T. cruzi-specific antibodies which are a biomarker for congenital Chagas disease (CCD). A screen-printed carbon electrode (SPCE) was modified with gold nanoparticles (AuNPs) and then functionalized with recombinant shed acute phase antigen (SAPA). The AuNPs/SPCE was characterized by scanning electron microscopy, cyclic voltammetry, and X-ray diffraction. The AuNPs provide an increased active surface area, high conductivity, and improved electrocatalytic characteristics, this resulting in a significant improvement of the detection limit. Following interaction between immobilized SAPA and the target antibodies in the serum sample, secondary antibody (an anti-human IgM-HRP conjugate) and solutions of H2O2 and 4-tert-butylcatechol were added and the enzymatic product was detected at −100 mV (vs. Ag/SPCE). The resulting current was proportional to the anti-T. cruzi IgM antibodies present in the sample and showed a linear trend from 10 to 200 ng mL−1 (R = 0.998). The detection limit was 3.03 ng mL−1. These values demonstrate that this electrochemical immunosensor can be used as an alternative serological method for diagnosis of congenital Chagas disease.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer Wien  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Cyclic Voltammetry  
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Horseradish Peroxidase  
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Recombinant Antigen  
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Sandwich Immunoassay  
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Scanning Electron Microscopy  
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Serology  
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X-Ray Diffraction  
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Otras Ciencias Químicas  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
An electrochemical immunosensor for anti-T. cruzi IgM antibodies, a biomarker for congenital Chagas disease, using a screen-printed electrode modified with gold nanoparticles and functionalized with shed acute phase antigen  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-09-20T13:21:57Z  
dc.identifier.eissn
1436-5073  
dc.journal.volume
183  
dc.journal.number
3  
dc.journal.pagination
1203-1210  
dc.journal.pais
Austria  
dc.journal.ciudad
Viena  
dc.description.fil
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.description.fil
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.description.fil
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.description.fil
Fil: Garcia, Carlos D.. Clemson University; Estados Unidos  
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Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.description.fil
Fil: Aranda, Pedro Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.journal.title
Mikrochimica Acta  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00604-016-1752-4  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00604-016-1752-4