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dc.contributor.author
Cabrera, Javier
dc.contributor.author
Saavedra, Ester
dc.contributor.author
del Rosario, Henoc
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Perdomo, Juan
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Loro, Juan F.
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Cifuente, Diego Alberto
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Tonn, Carlos Eugenio
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García, Celina
dc.contributor.author
Quintana, José
dc.contributor.author
Estévez, Francisco
dc.date.available
2018-09-21T20:17:52Z
dc.date.issued
2016-08
dc.identifier.citation
Cabrera, Javier; Saavedra, Ester; del Rosario, Henoc; Perdomo, Juan; Loro, Juan F.; et al.; Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species; Elsevier Ireland; Chemico-biological Interactions; 256; 8-2016; 220-227
dc.identifier.issn
0009-2797
dc.identifier.uri
http://hdl.handle.net/11336/60664
dc.description.abstract
Flavonoids have attracted great interest due to their possible anticancer activities. Here we investigated the antiproliferative activity of the flavonoids isolated from Baccharis scandens against human leukemia cell lines and found that the methoxyflavonoid gardenin B was the most cytotoxic compound against HL-60 and U-937 cells, showing IC50 values between 1.6 and 3.0 μM, but had no significant cytotoxic effects against quiescent or proliferating human peripheral blood mononuclear cells. These effects on viability were accompanied by the concentration- and time-dependent appearance of apoptosis as evidenced by DNA fragmentation, formation of apoptotic bodies and a sub-G1 ratio increase. Comparative studies with the best-studied bioflavonoid quercetin indicate that gardenin B is a more cytotoxic and more apoptotic inducer than quercetin. Cell death induced by gardenin B was associated with: (i) a significant induction of caspase-2, -3, -8 and -9 activities; (ii) cleavage of the initiator caspases (caspase-2, -8 and -9), of the executioner caspase-3, and of poly(ADP-ribose) polymerase; and (iii) a concentration-dependent reactive oxygen species generation. In conclusion, apoptosis induced by gardenin B is associated with activation of both the extrinsic and the intrinsic apoptotic pathways of cell death and occurs through a mechanism that is independent of the generation of reactive oxygen species.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Apoptosis
dc.subject
Baccharis Scandens
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Caspases
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Cytotoxicity
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Flavonoids
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Otras Ciencias Químicas
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Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-20T13:38:15Z
dc.journal.volume
256
dc.journal.pagination
220-227
dc.journal.pais
Irlanda
dc.description.fil
Fil: Cabrera, Javier. Universidad de Las Palmas de Gran Canaria; España
dc.description.fil
Fil: Saavedra, Ester. Universidad de Las Palmas de Gran Canaria; España
dc.description.fil
Fil: del Rosario, Henoc. Universidad de Las Palmas de Gran Canaria; España
dc.description.fil
Fil: Perdomo, Juan. Universidad de Las Palmas de Gran Canaria; España
dc.description.fil
Fil: Loro, Juan F.. Universidad de Las Palmas de Gran Canaria; España
dc.description.fil
Fil: Cifuente, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
dc.description.fil
Fil: Tonn, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
dc.description.fil
Fil: García, Celina. Universidad de La Laguna; España
dc.description.fil
Fil: Quintana, José. Universidad de Las Palmas de Gran Canaria; España
dc.description.fil
Fil: Estévez, Francisco. Universidad de Las Palmas de Gran Canaria; España
dc.journal.title
Chemico-biological Interactions
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.cbi.2016.07.016
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009279716302903


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