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dc.contributor.author
Cabrera, Javier  
dc.contributor.author
Saavedra, Ester  
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del Rosario, Henoc  
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Perdomo, Juan  
dc.contributor.author
Loro, Juan F.  
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Cifuente, Diego Alberto  
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Tonn, Carlos Eugenio  
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García, Celina  
dc.contributor.author
Quintana, José  
dc.contributor.author
Estévez, Francisco  
dc.date.available
2018-09-21T20:17:52Z  
dc.date.issued
2016-08  
dc.identifier.citation
Cabrera, Javier; Saavedra, Ester; del Rosario, Henoc; Perdomo, Juan; Loro, Juan F.; et al.; Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species; Elsevier Ireland; Chemico-biological Interactions; 256; 8-2016; 220-227  
dc.identifier.issn
0009-2797  
dc.identifier.uri
http://hdl.handle.net/11336/60664  
dc.description.abstract
Flavonoids have attracted great interest due to their possible anticancer activities. Here we investigated the antiproliferative activity of the flavonoids isolated from Baccharis scandens against human leukemia cell lines and found that the methoxyflavonoid gardenin B was the most cytotoxic compound against HL-60 and U-937 cells, showing IC50 values between 1.6 and 3.0 μM, but had no significant cytotoxic effects against quiescent or proliferating human peripheral blood mononuclear cells. These effects on viability were accompanied by the concentration- and time-dependent appearance of apoptosis as evidenced by DNA fragmentation, formation of apoptotic bodies and a sub-G1 ratio increase. Comparative studies with the best-studied bioflavonoid quercetin indicate that gardenin B is a more cytotoxic and more apoptotic inducer than quercetin. Cell death induced by gardenin B was associated with: (i) a significant induction of caspase-2, -3, -8 and -9 activities; (ii) cleavage of the initiator caspases (caspase-2, -8 and -9), of the executioner caspase-3, and of poly(ADP-ribose) polymerase; and (iii) a concentration-dependent reactive oxygen species generation. In conclusion, apoptosis induced by gardenin B is associated with activation of both the extrinsic and the intrinsic apoptotic pathways of cell death and occurs through a mechanism that is independent of the generation of reactive oxygen species.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Ireland  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Apoptosis  
dc.subject
Baccharis Scandens  
dc.subject
Caspases  
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Cytotoxicity  
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Flavonoids  
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Otras Ciencias Químicas  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-09-20T13:38:15Z  
dc.journal.volume
256  
dc.journal.pagination
220-227  
dc.journal.pais
Irlanda  
dc.description.fil
Fil: Cabrera, Javier. Universidad de Las Palmas de Gran Canaria; España  
dc.description.fil
Fil: Saavedra, Ester. Universidad de Las Palmas de Gran Canaria; España  
dc.description.fil
Fil: del Rosario, Henoc. Universidad de Las Palmas de Gran Canaria; España  
dc.description.fil
Fil: Perdomo, Juan. Universidad de Las Palmas de Gran Canaria; España  
dc.description.fil
Fil: Loro, Juan F.. Universidad de Las Palmas de Gran Canaria; España  
dc.description.fil
Fil: Cifuente, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina  
dc.description.fil
Fil: Tonn, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina  
dc.description.fil
Fil: García, Celina. Universidad de La Laguna; España  
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Fil: Quintana, José. Universidad de Las Palmas de Gran Canaria; España  
dc.description.fil
Fil: Estévez, Francisco. Universidad de Las Palmas de Gran Canaria; España  
dc.journal.title
Chemico-biological Interactions  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.cbi.2016.07.016  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009279716302903