Artículo
ICPMS analysis of proteins separated by Native-PAGE: Evaluation of metaloprotein profiles in human synovial fluid with acute and chronic arthritis
Moyano, Mario Franco
; Marino Repizo, Leonardo
; Tamashiro, Héctor; Villegas, Liliana Beatriz
; Acosta, Mariano
; Gil, Raul Andres
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Fecha de publicación:
07/2016
Editorial:
Elsevier Gmbh
Revista:
Journal of Trace Elements in Medicine and Biology
ISSN:
0946-672X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The role of trace elements bound to proteins in the etiology and pathogenesis of rheumatoid arthritis (RA) remains unclear. In this sense, the identification and detection of metalloproteins has a strong and growing interest. Metalloprotein studies are currently carried out by polyacrylamide gel electrophoresis (PAGE) associated to inductively coupled plasma mass spectrometry (ICPMS), and despite that complete information can be obtained for metals such as Fe, Cu and Zn, difficulties due to poor sensitivity for other trace elements such as Sn, As, etc, are currently faced. In the present work, a simple and fast method for the determination of trace metals bound to synovial fluid (SF) proteins was optimized. Proteins from SF (long and short-term RA) were separated in ten fractions by native PAGE, then dissolved in nitric acid and peroxide hydrogen, and analyzed by ICPMS. Fifteen metals were determined in each separated protein fraction (band). Adequate calibration of proteins molecular weight allowed stablishing which protein type were bound to different metals.
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Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Moyano, Mario Franco; Marino Repizo, Leonardo; Tamashiro, Héctor; Villegas, Liliana Beatriz; Acosta, Mariano; et al.; ICPMS analysis of proteins separated by Native-PAGE: Evaluation of metaloprotein profiles in human synovial fluid with acute and chronic arthritis; Elsevier Gmbh; Journal of Trace Elements in Medicine and Biology; 36; 7-2016; 44-51
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