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Artículo

Cellular actions of testosterone in vascular cells: mechanism independent of aromatization to estradiol

Campelo, Adrián EstebanIcon ; Cutini, Pablo HernanIcon ; Massheimer, Virginia LauraIcon
Fecha de publicación: 09/2012
Editorial: Elsevier Science Inc
Revista: Steroids
ISSN: 0039-128X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

In this work we investigated the role of testosterone on cellular processes involved in vascular disease, and whether these effects depend on its local conversion to estradiol. Cultures of rat aortic endothelial and smooth muscle cells in vitro treated with physiological concentrations of testosterone were employed. Testosterone rapidly increased endothelial nitric oxide production. To evaluate whether this non genomic action was dependent on testosterone aromatization we used an aromatase inhibitor. Anastrozole compound did not modify the fast increase in nitric oxide production elicited by testosterone. The hormonal effect was completely blocked by an androgen receptor antagonist (flutamide); meanwhile it wasńt modified by the presence of an estrogen receptor antagonist (ICI182780).The possibility of intracellular estradiol synthesis was ruled out when no differences were found in estradiol measurements performed in culture incubation medium from control and testosterone treated cells. The 5α-reductase inhibitor finasteride partially suppressed the enhancement in nitric oxide production, suggesting that the effect of testosterone was partially due to dihydrotestosterone conversion. Testosterone stimulated muscle cell proliferation independent of local conversion to estradiol. When cellular events that play key roles in vascular disease development were analyzed, testosterone prevented monocyte adhesion to endothelial cells induced by a proinflammatory stimulus (bacterial lipopolysaccharides), and prompted muscle cell migration in presence of a cell motility inducer. In summary, testosterone modulates vascular behavior through its direct action on vascular cells independent of aromatization to estradiol. The cellular actions exhibited by the steroid varied whether cells were under basal or inflammatory conditions.
Palabras clave: Aromatase , Nitric Oxide , Proliferation , Testosterone , Vascular
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/60656
DOI: http://dx.doi.org/10.1016/j.steroids.2012.05.008
URL: https://www.sciencedirect.com/science/article/pii/S0039128X12001821
Colecciones
Articulos(CCT - BAHIA BLANCA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - BAHIA BLANCA
Citación
Campelo, Adrián Esteban; Cutini, Pablo Hernan; Massheimer, Virginia Laura; Cellular actions of testosterone in vascular cells: mechanism independent of aromatization to estradiol; Elsevier Science Inc; Steroids; 77; 11; 9-2012; 1033-1040
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