Artículo
RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes
Recouvreux, Sol
; Grasso, Esteban Nicolas
; Echeverria, Pablo Christian
; Rocha Viegas, Luciana
; Castilla, Lucio Hernán; Schere Levy, Carolina Paula
; Tocci, Johanna Melisa
; Kordon, Edith Claudia
; Rubinstein, Natalia
Fecha de publicación:
12/2015
Editorial:
Impact Journals
Revista:
Oncotarget
ISSN:
1949-2553
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Abstract Runx1 participation in epithelial mammary cells is still under review. Emerging data indicates that Runx1 could be relevant for breast tumor promotion. However, to date no studies have specifically evaluated the functional contribution of Runx1 to control gene expression in mammary epithelial tumor cells. It has been described that Runx1 activity is defined by protein context interaction. Interestingly, Foxp3 is a breast tumor suppressor gene. Here we show that endogenous Runx1 and Foxp3 physically interact in normal mammary cells and this interaction blocks Runx1 transcriptional activity. Furthermore we demonstrate that Runx1 is able to bind to R-spondin 3 (RSPO3) and Gap Junction protein Alpha 1 (GJA1) promoters. This binding upregulates Rspo3 oncogene expression and downregulates GJA1 tumor suppressor gene expression in a Foxp3-dependent manner. Moreover, reduced Runx1 transcriptional activity decreases tumor cell migration properties. Collectively, these data provide evidence of a new mechanism for breast tumor gene expression regulation, in which Runx1 and Foxp3 physically interact to control mammary epithelial cell gene expression fate. Our work suggests for the first time that Runx1 could be involved in breast tumor progression depending on Foxp3 availability.
Palabras clave:
Oncologia
,
Mamario
,
Foxp3
,
Runx1
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Identificadores
Colecciones
Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Citación
Recouvreux, Sol; Grasso, Esteban Nicolas; Echeverria, Pablo Christian; Rocha Viegas, Luciana; Castilla, Lucio Hernán; et al.; RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes; Impact Journals; Oncotarget; 7; 6; 12-2015; 1-14
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