Regulation of spine density and morphology by IQGAP1 protein domains

IQGAP1 is a scaffolding protein that regulates spine number. We now show a differential role for IQGAP1 domains in spine morphogenesis, in which a region of the N-terminus that promotes Arp2/3-mediated actin polymerization and branching stimulates spine head formation while a region that binds to Cdc42 and Rac is required for stalk extension. Conversely, IQGAP1 rescues spine deficiency induced by expression of dominant negative Cdc42 by stimulating formation of stubby spines. Together, our observations place IQGAP1 as a crucial regulator of spine number and shape acting through the N-Wasp Arp2/3 complex, as well as upstream and downstream of Cdc42.

Palabras clave: Iqgap , Neuron , Spines

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

Identificadores

URI: http://hdl.handle.net/11336/604

DOI: http://dx.doi.org/10.1371/journal.pone.0056574

Colecciones

Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA

Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA

Citación

Jausoro, Ignacio; Mestres Lascano, Ivan; Quassollo Infanzon, Gonzalo Emiliano; Masseroni, Maria Luján; Heredia, María Florencia; Caceres, Alfredo Oscar; Regulation of spine density and morphology by IQGAP1 protein domains; Public Library Science; Plos One; 8; 2; 2-2013; e56574;

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