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dc.contributor.author
Wang, Huajin
dc.contributor.author
Quiroga, Ariel Dario
dc.contributor.author
Lehner, Richard
dc.date.available
2016-06-03T18:18:50Z
dc.date.issued
2013-10
dc.identifier.citation
Wang, Huajin; Quiroga, Ariel Dario; Lehner, Richard; Analysis of lipid droplets in hepatocytes; Elsevier; Methods In Cell Biology; 116; 10-2013; 107-127
dc.identifier.issn
0091-679X
dc.identifier.uri
http://hdl.handle.net/11336/6035
dc.description.abstract
Abstract The liver plays an important role in triacylglycerol (TG) metabolism. It can store large amounts of TG in cytosolic lipid droplets (CLDs), or it can package TG into very-low density lipoproteins (VLDL) that are secreted from the cell. TG packaged into VLDL is derived from TG stored within the endoplasmic reticulum in lumenal lipid droplets (LLDs). Therefore, the liver contains at least three kinds of LDs that differ in their protein composition, subcellular localization, and function. Hepatic LDs undergo tremendous changes in their size and protein composition depending on the energetic (fasting/feeding) and pathological (viral infection, nonalcoholic fatty liver disease, etc.) states. It is crucial to develop methodologies that allow the isolation and analyses of the various hepatic LDs in order to gain insight into the differential metabolism of these important lipid storage/transport particles in health and disease. Here, we present detailed protocols for the isolation and analysis of CLDs and LLDs and for monitoring CLD dynamics.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Cytosolic Lipid Droplets
dc.subject
Lumenal Lipid Droplets
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Bodipy Fatty Acids
dc.subject
Subcellular Fractionation
dc.subject.classification
Fisiología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Analysis of lipid droplets in hepatocytes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-06-01T13:33:08Z
dc.journal.volume
116
dc.journal.pagination
107-127
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Burlington
dc.description.fil
Fil: Wang, Huajin. University Of Yale. Yale School Of Forestry & Environmental Studies; Estados Unidos
dc.description.fil
Fil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
dc.description.fil
Fil: Lehner, Richard. University Of Alberta; Canadá
dc.journal.title
Methods In Cell Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/B9780124080515000073
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/B978-0-12-408051-5.00007-3
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1016/B978-0-12-408051-5.00007-3


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