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dc.contributor.author
Balado, Miguel
dc.contributor.author
Souto, Alba
dc.contributor.author
Vences, Ana
dc.contributor.author
Careaga Quiroga, Valeria Pilar
dc.contributor.author
Valderrama, Katherine
dc.contributor.author
Segade, Yuri
dc.contributor.author
Rodríguez, Jaime
dc.contributor.author
Osorio, Carlos R.
dc.contributor.author
Jiménez, Carlos
dc.contributor.author
Lemos, Manuel L.
dc.date.available
2018-09-19T17:15:00Z
dc.date.issued
2015-12
dc.identifier.citation
Balado, Miguel; Souto, Alba; Vences, Ana; Careaga Quiroga, Valeria Pilar; Valderrama, Katherine; et al.; Two Catechol Siderophores, Acinetobactin and Amonabactin, Are Simultaneously Produced by Aeromonas salmonicida subsp. salmonicida Sharing Part of the Biosynthetic Pathway; American Chemical Society; ACS Chemical Biology; 10; 12; 12-2015; 2850-2860
dc.identifier.issn
1554-8929
dc.identifier.uri
http://hdl.handle.net/11336/60237
dc.description.abstract
The iron uptake mechanisms based on siderophore synthesis used by the fish pathogen Aeromonas salmonicida subsp. salmonicida are still not completely understood, and the precise structure of the siderophore(s) is unknown. The analysis of genome sequences revealed that this bacterium possesses two gene clusters putatively involved in the synthesis of siderophores. One cluster is a candidate to encode the synthesis of acinetobactin, the siderophore of the human pathogen Acinetobacter baumannii, while the second cluster shows high similarity to the genes encoding amonabactin synthesis in Aeromonas hydrophila. Using a combination of genomic analysis, mutagenesis, biological assays, chemical purification, and structural determination procedures, here we demonstrate that most A. salmonicida subsp. salmonicida strains produce simultaneously the two siderophores, acinetobactin and amonabactin. Interestingly, the synthesis of both siderophores relies on a single copy of the genes encoding the synthesis of the catechol moiety (2,3-dihydroxybenzoic acid) and on one encoding a phosphopantetheinyl transferase. These genes are present only in the amonabactin cluster, and a single mutation in any of them abolishes production of both siderophores. We could also demonstrate that some strains, isolated from fish raised in seawater, produce only acinetobactin since they present a deletion in the amonabactin biosynthesis gene amoG. Our study represents the first evidence of simultaneous production of acinetobactin and amonabactin by a bacterial pathogen and reveals the plasticity of bacterial genomes and biosynthetic pathways. The fact that the same siderophore is produced by unrelated pathogens highlights the importance of these systems and their interchangeability between different bacteria.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Aeromonas
dc.subject
Siderophores
dc.subject
Iron Uptake
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Two Catechol Siderophores, Acinetobactin and Amonabactin, Are Simultaneously Produced by Aeromonas salmonicida subsp. salmonicida Sharing Part of the Biosynthetic Pathway
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-04T19:02:14Z
dc.journal.volume
10
dc.journal.number
12
dc.journal.pagination
2850-2860
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Balado, Miguel. Universidad de Santiago de Compostela; España
dc.description.fil
Fil: Souto, Alba. Universidad da Coruña; España
dc.description.fil
Fil: Vences, Ana. Universidad de Santiago de Compostela; España
dc.description.fil
Fil: Careaga Quiroga, Valeria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad da Coruña; España
dc.description.fil
Fil: Valderrama, Katherine. Universidad da Coruña; España
dc.description.fil
Fil: Segade, Yuri. Universidad da Coruña; España
dc.description.fil
Fil: Rodríguez, Jaime. Universidad da Coruña; España
dc.description.fil
Fil: Osorio, Carlos R.. Universidad de Santiago de Compostela; España
dc.description.fil
Fil: Jiménez, Carlos. Universidad da Coruña; España
dc.description.fil
Fil: Lemos, Manuel L.. Universidad de Santiago de Compostela; España
dc.journal.title
ACS Chemical Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1021/acschembio.5b00624
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acschembio.5b00624
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