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dc.contributor.author
Aquino Esperanza, Jose A.
dc.contributor.author
Aguirre, María Victoria
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Aispuru, Gualberto R.
dc.contributor.author
Lettieri, Carolina N.
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Juaristi, Julian Antonio
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Alvarez, Mirta Alba
dc.contributor.author
Brandan, Nora Cristina
dc.date.available
2018-09-18T22:44:52Z
dc.date.issued
2008-10
dc.identifier.citation
Aquino Esperanza, Jose A.; Aguirre, María Victoria; Aispuru, Gualberto R.; Lettieri, Carolina N.; Juaristi, Julian Antonio; et al.; In vivo 5-flourouracil-induced apoptosis on murine thymocytes: Involvement of FAS, Bax and Caspase3; Springer; Cell Biology And Toxicology; 24; 10-2008; 411-422
dc.identifier.issn
0742-2091
dc.identifier.uri
http://hdl.handle.net/11336/60203
dc.description.abstract
Apoptosis is a highly regulated and programmed cell breakdown process characterized by numerous changes. It was reported as the major mechanism of anticancer drug-induced cells death. Unfortunately, many of these drugs are non-specific and cause severe side effects. The effects of 5-fluorouracil (5-FU) on the apoptotic events in normal murine thymus were evaluated using an in vivo model. A single dose of 5-FU (150 mg/kg ip) was injected to CF-1 mice. A multiparametric analysis of thymic weight, cellularity, viability, architectural organization, apoptosis, DNA fragmentation, and the expression of several apoptotic proteins was evaluated in 10 days time-course study post-5-FU dosing. Total organ weights, thymocyte counts, and cell viabilities diminished drastically from the second day. The thymus architecture assessed through electron scanning microscopy revealed deep alterations and the lost of cell-cell contact between the first and the third days. DNA fragmentation and apoptotic indexes (May Grünwald Giemsa staining, double fluorescent dyes, and TdT-mediated dUTP nick-end labeling assay) revealed that cell death was maximal on the second day (three times over control). Furthermore, the pro-apoptotic proteins FAS and Bax were strongly up-regulated during the first 2 days. The aforementioned morphological and biochemical changes were also accompanied within the same period by caspase 3 activation. This study revealed that in vivo apoptosis in normal thymus after 5-FU administration is related to FAS, Bax, and Caspase 3 co-expressions under the current experimental conditions, these findings, therefore, contribute to a new insight into the molecular mechanisms involved during 5-FU administration upon the thymus and the possible events committed in the lymphophenia associated with chemotherapy.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
5-FU
dc.subject
MURINE THYMUS
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APOPTOSIS
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FAS
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BAX
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CASPASE 3
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Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
In vivo 5-flourouracil-induced apoptosis on murine thymocytes: Involvement of FAS, Bax and Caspase3
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-18T14:05:15Z
dc.journal.number
24
dc.journal.pagination
411-422
dc.journal.pais
Alemania
dc.description.fil
Fil: Aquino Esperanza, Jose A.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina
dc.description.fil
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina
dc.description.fil
Fil: Aispuru, Gualberto R.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina
dc.description.fil
Fil: Lettieri, Carolina N.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina
dc.description.fil
Fil: Juaristi, Julian Antonio. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina
dc.description.fil
Fil: Alvarez, Mirta Alba. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
dc.description.fil
Fil: Brandan, Nora Cristina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
dc.journal.title
Cell Biology And Toxicology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10565-008-9056-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10565-008-9056-z
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