Artículo
B-esterase determination and organophosphate insecticide inhibitory effects in JEG-3 trophoblasts
Espinoza, Marlon; Rivero Osimani, Valeria Leticia
; Sánchez, Victoria Guadalupe
; Rosenbaum, Enrique Arturo; Guiñazu Alaniz, Natalia Lorena
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
04/2016
Editorial:
Pergamon-Elsevier Science Ltd
Revista:
Toxicology in Vitro
ISSN:
0887-2333
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The placenta and trophoblasts express several B-esterases. This family includes acethylcholinesterase (AChE), carboxylesterase (CES) and butyrylcholinesterase (BChE), which are important targets of organophosphate insecticide (OP) toxicity. To better understand OP effects on trophoblasts, B-esterase basal activity and kinetic behavior were studied in JEG-3 choriocarcinoma cell cultures. Effects of the OP azinphos-methyl (Am) and chlorpyrifos (Cp) on cellular enzyme activity were also evaluated. JEG-3 cells showed measurable activity levels of AChE and CES, while BChE was undetected. Recorded Km for AChE and CES were 0.33 and 0.26 mM respectively. Native gel electrophoresis and RT-PCR analysis demonstrated CES1 and CES2 isoform expression. Cells exposed for 4 and 24 h to the OP Am or Cp, showed a differential CES and AChE inhibition profiles. Am inhibited CES and AChE at 4 h treatment while Cp showed the highest inhibition profile at 24 h. Interestingly, both insecticides differentially affected CES1 and CES2 activities. Results demonstrated that JEG-3 trophoblasts express AChE, CES1 and CES2. B-esterase enzymes were inhibited by in vitro OP exposure, indicating that JEG-3 cells metabolization capabilities include phase I enzymes, able to bioactivate OP. In addition, since CES enzymes are important for medicinal drug activation/deactivation, OP exposure may interfere with trophoblast CES metabolization, probably being relevant in a co-exposure scenario during pregnancy.
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Articulos(CCT - PATAGONIA NORTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - PATAGONIA NORTE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - PATAGONIA NORTE
Articulos(CITAAC)
Articulos de CENTRO DE INVESTIGACIONES EN TOXICOLOGIA AMBIENTAL Y AGROBIOTECNOLOGIA DEL COMAHUE
Articulos de CENTRO DE INVESTIGACIONES EN TOXICOLOGIA AMBIENTAL Y AGROBIOTECNOLOGIA DEL COMAHUE
Citación
Espinoza, Marlon; Rivero Osimani, Valeria Leticia; Sánchez, Victoria Guadalupe; Rosenbaum, Enrique Arturo; Guiñazu Alaniz, Natalia Lorena; B-esterase determination and organophosphate insecticide inhibitory effects in JEG-3 trophoblasts; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 32; 4-2016; 190-197
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