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dc.contributor.author
Tomat, David Damian
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dc.contributor.author
Balague, Claudia Elisabeht
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dc.contributor.author
Aquili, Virginia
dc.contributor.author
Verdini, Roxana Andrea
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dc.contributor.author
Quiberoni, Andrea del Lujan
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dc.date.available
2018-09-17T22:04:01Z
dc.date.issued
2018-02
dc.identifier.citation
Tomat, David Damian; Balague, Claudia Elisabeht; Aquili, Virginia; Verdini, Roxana Andrea; Quiberoni, Andrea del Lujan; Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings; Wiley Blackwell Publishing, Inc; International Journal of Food Science and Technology; 53; 2; 2-2018; 533-540
dc.identifier.issn
0950-5423
dc.identifier.uri
http://hdl.handle.net/11336/60028
dc.description.abstract
Phages are potentially useful as antimicrobial agents in food-processing environments, provided they can remain active upon extended storage and in the presence of sanitisers. Survival of six phages lytic against enteropathogenic (EPEC) and shiga-toxigenic (STEC) Escherichia coli strains was assessed upon storage at 4 °C, −20 °C and −70 °C in phosphate-buffered-saline (PBS) and Tris-magnesium-gelatine buffer (TMG) for up to 1 year. The phages were also exposed to ethanol, sodium hypochlorite, peracetic acid, quaternary ammonium chloride (biocide A), hydrogen peroxide/peracetic acid/peroctanoic acid (biocide B), p-toluensulfonchloroamide (biocide D) and alkaline chloride foam (biocide C). Plaque-forming units remained unchanged when the phages were stored at 4 °C in both buffers tested, but decreased by 3.5 and 5.7 log10 PFU when stored in PBS at −20 and −70 °C, respectively. Moreover, TMG seems to be the most protective suspension medium with decreasing temperature because a 1−log10 PFU reduction was observed at −20 and −70 °C. Incubation in 100% ethanol for 24 h reduced plaque counts only by 2.5 log10 PFU. In 10 ppm of sodium hypochlorite and in biocide B (0.13%), the counts decreased by ~5 and ~6 log10 PFU after 15 min. Finally, biocide A and D reduced counts by 4 and 2–4 log10 PFU after 30 min and 8 h of incubation, respectively. Phages were completely inactivated only by peracetic acid and biocides C and E. Therefore, the phages evaluated might be potentially applied together with classical sanitisers such as ethanol and industrial biocides A, B and D, in disinfection programs against pathogenic STEC and EPEC strains.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bacteriophage
dc.subject
Escherichia Coli
dc.subject
Phage Viability
dc.subject
Sanitiser
dc.subject.classification
Alimentos y Bebidas
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dc.subject.classification
Otras Ingenierías y Tecnologías
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-14T13:25:40Z
dc.journal.volume
53
dc.journal.number
2
dc.journal.pagination
533-540
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Tomat, David Damian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina
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Fil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina
dc.description.fil
Fil: Aquili, Virginia. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Verdini, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
dc.description.fil
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
dc.journal.title
International Journal of Food Science and Technology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/ijfs.13626
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