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dc.contributor.author
Dhiman, Monisha
dc.contributor.author
Coronado, Yun A.
dc.contributor.author
Vallejo, Cecilia K.
dc.contributor.author
Petersen, John R.
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Ejilemele, Adetoum
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Nuñez, Sonia
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Zago, María Paola
dc.contributor.author
Spratt, Heidi
dc.contributor.author
Garg, Nisha Jain
dc.date.available
2015-06-03T19:55:55Z
dc.date.issued
2013-08
dc.identifier.citation
Dhiman, Monisha; Coronado, Yun A.; Vallejo, Cecilia K.; Petersen, John R.; Ejilemele, Adetoum; Nuñez, Sonia; Zago, María Paola; Spratt, Heidi; Garg, Nisha Jain; Innate immune responses and antioxidant/oxidant imbalance are major determinants of human Chagas disease.; Public Library Science; Plos Neglected Tropical Diseases; 8; 8-2013; 2364-2364;
dc.identifier.issn
1935-2735
dc.identifier.uri
http://hdl.handle.net/11336/596
dc.description.abstract
We investigated the pathological and diagnostic role of selected markers of inflammation, oxidant/antioxidant status, and cellular injury in human Chagas disease. METHODS: Seropositive/chagasic subjects characterized as clinically-symptomatic or clinically-asymptomatic (n = 116), seronegative/cardiac subjects (n = 102), and seronegative/healthy subjects (n = 45) were analyzed for peripheral blood biomarkers. RESULTS: Seropositive/chagasic subjects exhibited an increase in sera or plasma levels of myeloperoxidase (MPO, 2.8-fold), advanced oxidation protein products (AOPP, 56%), nitrite (5.7-fold), lipid peroxides (LPO, 12-17-fold) and malondialdehyde (MDA, 4-6-fold); and a decline in superoxide dismutase (SOD, 52%) and glutathione (GSH, 75%) contents. Correlation analysis identified a significant (p<0.001) linear relationship between inflammatory markers (AOPP/nitrite: r = 0.877), inflammation and antioxidant/oxidant status (AOPP/glutathione peroxidase (GPX): r = 0.902, AOPP/GSH: r = 0.806, Nitrite/GPX: 0.773, Nitrite/LPO: 0.805, MDA/MPO: 0.718), and antioxidant/oxidant levels (GPX/MDA: r = 0.768) in chagasic subjects. Of these, MPO, LPO and nitrite biomarkers were highly specific and sensitive for distinguishing seropositive/chagasic subjects from seronegative/healthy controls (p<0.001, training and fitting AUC/ROC >0.95). The MPO (r = 0.664) and LPO (r = 0.841) levels were also correlated with clinical disease state in chagasic subjects (p<0.001). Seronegative/cardiac subjects exhibited up to 77% decline in SOD, 3-5-fold increase in LPO and glutamate pyruvate transaminase (GPT) levels, and statistically insignificant change in MPO, AOPP, MDA, GPX, GSH, and creatine kinase (CK) levels. CONCLUSIONS: The interlinked effects of innate immune responses and antioxidant/oxidant imbalance are major determinants of human Chagas disease. The MPO, LPO and nitrite are excellent biomarkers for diagnosing seropositive/chagasic subjects, and MPO and LPO levels have potential utility in identifying clinical severity of Chagas disease
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Oxidative Stress
dc.subject
Biomarkers
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Chagas Disease
dc.subject.classification
Ciencias Médicas y de la Salud
dc.subject.classification
Ciencias de la Salud
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Parasitología
dc.title
Innate immune responses and antioxidant/oxidant imbalance are major determinants of human Chagas disease.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.pagination
2364-2364
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Dhiman, Monisha. University Of Texas Medical Branch. Department Of Microbiology & Immunology And Pathology; United State of America;
dc.description.fil
Fil: Coronado, Yun A.. University Of Texas Medical Branch. Department Of Microbiology & Immunology And Pathology; United State of America;
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Fil: Vallejo, Cecilia K.. University Of Texas Medical Branch. Department Of Microbiology & Immunology And Pathology; United State of America;
dc.description.fil
Fil: Petersen, John R.. University of Texas Medical Branch. Department of Pathology; United States of America;
dc.description.fil
Fil: Ejilemele, Adetoum. University of Texas Medical Branch. Department of Pathology; United States of America;
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Fil: Nuñez, Sonia. Hospital Público de Gestión Descentralizada San Bernardo (HPGDSA); Argentina;
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Fil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Salta. Instituto de Patologia Experimental; Argentina;
dc.description.fil
Fil: Spratt, Heidi. Departments of Biochemistry and Molecular Biology and Preventive Medicine and Community Health. University of Texas Medical Branch; United States of America;
dc.description.fil
Fil: Garg, Nisha Jain. University of Texas Medical Branch. Department of Pathology; United States of America;
dc.journal.title
Plos Neglected Tropical Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pntd.0002364
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738450/