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dc.contributor.author
Steiner, Neusa
dc.contributor.author
Farias-Soares, Francine L.
dc.contributor.author
Schmidt, Éder C.
dc.contributor.author
Pereira, Maria L. T.
dc.contributor.author
Scheid, Bruna
dc.contributor.author
Rogge-Renner, Gladys D.
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Bouzon, Zenilda L.
dc.contributor.author
Schmidt, Daniela
dc.contributor.author
Maldonado, Sara Beatriz
dc.contributor.author
Guerra, Miguel P.
dc.date.available
2018-09-13T18:58:08Z
dc.date.issued
2016-03
dc.identifier.citation
Steiner, Neusa; Farias-Soares, Francine L.; Schmidt, Éder C.; Pereira, Maria L. T.; Scheid, Bruna; et al.; Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze; Springer Wien; Protoplasma; 253; 2; 3-2016; 487-501
dc.identifier.issn
0033-183X
dc.identifier.uri
http://hdl.handle.net/11336/59589
dc.description.abstract
Somatic embryogenesis is a morphogenetic route useful for the study of embryonic development, as well as the large-scale propagation of endangered species, such as the Brazilian pine (Araucaria angustifolia). In the present study, we investigated the morphological and ultrastructural organization of A. angustifolia somatic embryo development by means of optical and electron microscopy. The proembryogenic stage was characterized by the proliferation of proembryogenic masses (PEMs), which are cellular aggregates composed of embryogenic cells (ECs) attached to suspensor-like cells (SCs). PEMs proliferate through three developmental stages, PEM I, II, and III, by changes in the number of ECs and SCs. PEM III-to-early somatic embryo (SE) transition was characterized by compact clusters of ECs growing out of PEM III, albeit still connected to it by SCs. Early SEs showed a dense globular embryonic mass (EM) and suspensor region (SR) connected by embryonic tube cells (TCs). By comparison, early somatic and zygotic embryos showed similar morphology. ECs are round with a large nucleus, nucleoli, and many cytoplasmic organelles. In contrast, TCs and SCs are elongated and vacuolated with cellular dismantling which is associated with programmed cell death of SCs. Abundant starch grains were observed in the TCs and SCs, while proteins were more abundant in the ECs. Based on the results of this study, a fate map of SE development in A. angustifolia is, for the first time, proposed. Additionally, this study shows the cell biology of SE development of this primitive gymnosperm which may be useful in evolutionary studies in this area.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Wien
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Conifers
dc.subject
Histochemistry
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Pluripotency
dc.subject
Somatic Embryogenesis
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Suspensor Cell
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-13T13:14:13Z
dc.journal.volume
253
dc.journal.number
2
dc.journal.pagination
487-501
dc.journal.pais
Austria
dc.journal.ciudad
Viena
dc.description.fil
Fil: Steiner, Neusa. Universidade Federal de Santa Catarina; Brasil
dc.description.fil
Fil: Farias-Soares, Francine L.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil
Fil: Schmidt, Éder C.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil
Fil: Pereira, Maria L. T.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil
Fil: Scheid, Bruna. Universidade Federal de Santa Catarina; Brasil
dc.description.fil
Fil: Rogge-Renner, Gladys D.. Universidade Da Regiao de Joinville; Brasil. Universidade Federal de Santa Catarina; Brasil
dc.description.fil
Fil: Bouzon, Zenilda L.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil
Fil: Schmidt, Daniela. Universidade Federal de Santa Catarina; Brasil
dc.description.fil
Fil: Maldonado, Sara Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
dc.description.fil
Fil: Guerra, Miguel P.. Universidade Federal de Santa Catarina; Brasil
dc.journal.title
Protoplasma
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s00709-015-0827-0
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00709-015-0827-0
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