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Artículo

Role of FSH glycan structure in the regulation of Sertoli cell inhibin production

Andreone, LuzIcon ; Ambao, Veronica AnaIcon ; Pellizzari, Eliana HerminiaIcon ; Loreti, Rosana NazarethIcon ; Cigorraga, Selva BeatrizIcon ; Campo, Stella MarisIcon
Fecha de publicación: 08/2017
Editorial: BioScientifica
Revista: Reproduction
ISSN: 1470-1626
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Variations in follicle-stimulating hormone (FSH) carbohydrate composition and structure are associated with important structural and functional changes in Sertoli cells (SCs) during sexual maturation. The aim of the present study was to investigate the impact of FSH oligosaccharide structure and its interaction with gonadal factors on the regulation of monomeric and dimeric inhibin production at different maturation stages of the SC. Recombinant human FSH (rhFSH) glycosylation variants were isolated according to their sialylation degree (AC and BA) and complexity of oligosaccharides (CO and HY). Native rhFSH stimulated inhibin α-subunit (Pro-αC) but did not show any effect on inhibin B (INHB) production in immature SCs isolated from 8-day-old rats. Activin A stimulated INHB and had a synergistic effect on FSH to stimulate Pro-αC. The less acidic/sialylated rhFSH charge analogues, BA, were the only charge analogue mix that stimulated INHB as well as the most potent stimulus for Pro-αC production. Native rhFSH stimulated both Pro-αC and INHB in SCs at a more advanced maturation stage, isolated from 20-day-old rats. In these cells, all rhFSH glycosylation variants increased INHB and Pro-αC production, even in the presence of growth factors. The BA preparation exerted a more marked stimulatory effect on INHB and Pro-αC than the AC. Glycoforms bearing high mannose and hybrid-type oligosaccharides, HY, stimulated INHB and Pro-αC more effectively than those bearing complex oligosaccharides, CO, even in the presence of gonadal growth factors. These findings demonstrate the modulatory effect of FSH oligosaccharide structure on the regulation of inhibin production in the male gonad.
Palabras clave: Inhibin B , Fsh Glycoforms , Sertoli Cell , Activin A
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/59154
DOI: http://dx.doi.org/10.1530/REP-17-0393
URL: https://rep.bioscientifica.com/view/journals/rep/154/5/REP-17-0393.xml
Colecciones
Articulos(CEDIE)
Articulos de CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Articulos(IBIOBA - MPSP)
Articulos de INST. D/INV.EN BIOMED.DE BS AS-CONICET-INST. PARTNER SOCIEDAD MAX PLANCK
Citación
Andreone, Luz; Ambao, Veronica Ana; Pellizzari, Eliana Herminia; Loreti, Rosana Nazareth; Cigorraga, Selva Beatriz; et al.; Role of FSH glycan structure in the regulation of Sertoli cell inhibin production; BioScientifica; Reproduction; 154; 5; 8-2017; 711-721
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