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dc.contributor.author
Abeijon Mukdsi, Maria Claudia
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dc.contributor.author
Argañaraz Martínez, Fernando Eloy
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dc.contributor.author
Perez Chaia, Adriana Beatriz
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dc.contributor.author
Medina, Roxana Beatriz
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dc.date.available
2018-09-10T20:55:13Z
dc.date.issued
2016-04-19
dc.identifier.citation
Abeijon Mukdsi, Maria Claudia; Argañaraz Martínez, Fernando Eloy; Perez Chaia, Adriana Beatriz; Medina, Roxana Beatriz; Feruloyl esterase activity is influenced by bile, probiotic intestinal adhesion and milk fat; Wageningen Academic Publishers; Beneficial Microbes; 7; 4; 19-4-2016; 597-607
dc.identifier.issn
1876-2883
dc.identifier.uri
http://hdl.handle.net/11336/58985
dc.description.abstract
Cinnamoyl esterases (CE) are microbial and mammalian intestinal enzymes able to release antioxidant hydroxycinnamic acids from their non-digestible ester-linked forms naturally present in vegetable foods. Previous findings showed that oral administration of Lactobacillus fermentum CRL1446 increased intestinal CE activity and improved oxidative status in mice. The aim of this work was to evaluate the in vitro CE activity of L. fermentum CRL1446 and the effect of bile on this activity, as well as strain resistance to simulated gastrointestinal tract (GIT) conditions and its ability to adhere to intestinal epithelium and influence its basal CE activity. L. fermentum CRL1446 and L. fermentum ATCC14932 (positive control for CE activity) were able to hydrolyse different synthetic hydroxycinnamates, with higher specificity toward methyl ferulate (3,853.73 and 899.19 U/g, respectively). Feruloyl esterase (FE) activity was mainly intracellular in L. fermentum CRL1446 and cell-surface associated in L. fermentum ATCC14932. Both strains tolerated simulated GIT conditions and were able to adhere ex vivo to intestinal epithelium. Pre-incubation of L. fermentum strains with bile increased FE activity in both whole cells and supernatants (~2-fold), compared to controls, suggesting that cells were permeabilised by bile, allowing more substrate to enter the cell and/or leakage of FE enzymes. Three-fold higher FE activities were detected in intestinal tissue fragments with adhered L. fermentum CRL1446 cells compared to control fragments (without bacteria), indicating that this strain provides exogenous FE activity and could stimulate esterase activity in the intestinal mucosa. Finally, we found that milk fat had a negative effect on FE activity of intestinal tissue, in absence or presence of adhered L. fermentum. These results help explaining the increase in intestinal FE activity previously observed in mice fed with L. fermentum CRL1446, and support the potential use of this strain for the development of new functional foods directed to oxidative stress-related ailments.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wageningen Academic Publishers
dc.rights
info:eu-repo/semantics/openAccess
dc.rights
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Antioxidant Hydroxycinnamic Acids
dc.subject
Functional Foods
dc.subject
Intestinal Esterase Activity
dc.subject
Lactobacillus Fermentum
dc.subject
Probiotics
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Feruloyl esterase activity is influenced by bile, probiotic intestinal adhesion and milk fat
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-21T16:45:42Z
dc.identifier.eissn
1876-2891
dc.journal.volume
7
dc.journal.number
4
dc.journal.pagination
597-607
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Wageningen
dc.description.fil
Fil: Abeijon Mukdsi, Maria Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina
dc.description.fil
Fil: Argañaraz Martínez, Fernando Eloy. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: Medina, Roxana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
dc.journal.title
Beneficial Microbes
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3920/BM2015.0197
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.3920/BM2015.0197
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