Artículo
The N-terminal pre-A region of Mycobacterium tuberculosis 2/2HbN promotes NO-dioxygenase activity
Pesce, Alessandra; Bustamante, Juan Pablo
; Bidon Chanal, Axel; Boechi, Leonardo
; Estrin, Dario Ariel
; Luque, Francisco Javier; Sebilo, Anne; Guertin, Michel; Bolognesi, Martino; Ascenzi, Paolo; Nardini, Marco
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
01/2016
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Febs Journal
ISSN:
1742-464X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A unique defense mechanisms by which Mycobacterium tuberculosis protects itself from nitrosative stress is based on the O2-dependent NO-dioxygenase (NOD) activity of truncated hemoglobin 2/2HbN (Mt2/2HbN). The NOD activity largely depends on the efficiency of ligand migration to the heme cavity through a two-tunnel (long and short) system; recently, it was also correlated with the presence at the Mt2/2HbN N-terminus of a short pre-A region, not conserved in most 2/2HbNs, whose deletion results in a drastic reduction of NO scavenging. In the present study, we report the crystal structure of Mt2/2HbN-ΔpreA, lacking the pre-A region, at a resolution of 1.53 Å. We show that removal of the pre-A region results in long range effects on the protein C-terminus, promoting the assembly of a stable dimer, both in the crystals and in solution. In the Mt2/2HbN-ΔpreA dimer, access of heme ligands to the short tunnel is hindered. Molecular dynamics simulations show that the long tunnel branch is the only accessible pathway for O2-ligand migration to/from the heme, and that the gating residue Phe(62)E15 partly restricts the diameter of the tunnel. Accordingly, kinetic measurements indicate that the kon value for peroxynitrite isomerization by Mt2/2HbN-ΔpreA-Fe(III) is four-fold lower relative to the full-length protein, and that NO scavenging by Mt2/2HbN-ΔpreA-Fe(II)-O2 is reduced by 35-fold. Therefore, we speculate that Mt2/2HbN evolved to host the pre-A region as a mechanism for preventing dimerization, thus reinforcing the survival of the microorganism against the reactive nitrosative stress in macrophages. Database Coordinates and structure factors have been deposited in the Protein Data Bank under accession number 5AB8. Removal of the pre-A region in M. tuberculosis 2/2HbN (Mt2/2HbN-ΔpreA) results in dimerization and in a reduced access to the heme pocket. Kinetic measurements indicate a 4-fold decrease in kon for peroxynitrite isomerization and a 35-fold decrease in NO-scavenging relative to full-length Mt2/2HbN. Thus, the pre-A region might be involved in reinforcing survival of the microorganism against nitrosative stress in macrophages.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INQUIMAE)
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Citación
Pesce, Alessandra; Bustamante, Juan Pablo; Bidon Chanal, Axel; Boechi, Leonardo; Estrin, Dario Ariel; et al.; The N-terminal pre-A region of Mycobacterium tuberculosis 2/2HbN promotes NO-dioxygenase activity; Wiley Blackwell Publishing, Inc; Febs Journal; 283; 2; 1-2016; 305-322
Compartir
Altmétricas