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Artículo

Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs

Clausse, MaríaIcon ; Díaz, Alejandra GracielaIcon ; Pardo, Romina PaolaIcon ; Zylberman, VanesaIcon ; Goldbaum, Fernando AlbertoIcon ; Estein, Silvia MarcelaIcon
Fecha de publicación: 02/2017
Editorial: Elsevier Science
Revista: Veterinary Immunology And Immunopathology
ISSN: 0165-2427
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Enfermedades Infecciosas

Resumen

Polymeric antigen BLSOmp31 is an immunogenic vaccine candidate that confers protection against Brucella canis in mice. In this preliminary study, the immunogenicity and safety of BLSOmp31 adsorbed to aluminum hydroxide gel (BLSOmp31-AH) were evaluated in Beagle dogs. In addition, the potential to elicit serum antibodies with complement-dependent bactericidal activity and/or to enhance phagocytosis by neutrophils were analyzed. Dogs were immunized three times with BLSOmp31-AH by subcutaneous route, followed by an annual booster. The vaccine elicited specific antibodies 3 weeks after the first immunization. Annual booster induced comparable antibody response as the primary series. Humoral immune response stimulated by BLSOmp31-AH did not interfere with routine agglutination test for canine brucellosis. Antibodies demonstrated a high complement-dependent bactericidal activity against B. canis. Moreover, opsonization by immune serum not only stimulated binding and uptake of the bacteria by neutrophils but effectively enhanced the destruction of B. canis. Specific IgG was detected in 3/4 immunized dogs in preputial secretions. The antibody profile corresponded to a marked Th2 response, since IgG1 prevailed over IgG2 and cellular immune response was not detected in vitro or in vivo. These results require further evaluation in larger field studies to establish the full prophylactic activity of BLSOmp31 against canine brucellosis.
Palabras clave: Aluminum Hydroxide , Bacteriolytic Antibodies , Blsomp31 , Canine Brucellosis , Dog , Opsonic Antibodies
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/58616
DOI: https://doi.org/10.1016/j.vetimm.2016.11.004
URL: https://www.sciencedirect.com/science/article/pii/S0165242716303257
Colecciones
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Clausse, María; Díaz, Alejandra Graciela; Pardo, Romina Paola; Zylberman, Vanesa; Goldbaum, Fernando Alberto; et al.; Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs; Elsevier Science; Veterinary Immunology And Immunopathology; 184; 2-2017; 36-41
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