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dc.contributor.author
Juárez Tomás, María Silvina
dc.contributor.author
Bru Chauve, Elena Magdalena
dc.contributor.author
Wiese, Birgitt
dc.contributor.author
Pesce de Ruiz Holgado, Aida Argentina
dc.contributor.author
Nader, Maria Elena Fatima
dc.date.available
2018-09-06T18:59:37Z
dc.date.issued
2002-09
dc.identifier.citation
Juárez Tomás, María Silvina; Bru Chauve, Elena Magdalena; Wiese, Birgitt; Pesce de Ruiz Holgado, Aida Argentina; Nader, Maria Elena Fatima; Influence of pH, temperature and culture media on the growth and bacteriocin production by vaginal Lactobacillus salivarius CRL 1328; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 93; 4; 9-2002; 714-724
dc.identifier.issn
1364-5072
dc.identifier.uri
http://hdl.handle.net/11336/58579
dc.description.abstract
Aims: To study the influence of pH, temperature and culture medium on the growth and bacteriocin production by vaginal Lactobacillus salivarius subsp. salivarius CRL 1328. Methods and Results: The study was performed using a complete factorial experimental design. Lactobacillus salivarius was cultivated in LAPTg and MRS broths, adjusted to specific initial pH, and at different temperatures of incubation. The growth, which was evaluated by the Gompertz model, was higher in MRS broth than in LAPTg broth. The initial pH of the culture medium and the temperature had a dramatic effect on the production of bacteriocin. The optimal conditions for bacteriocin production were different to those for optimal growth. The decrease in the pH of the culture medium was parallel to the growth; pH had similar final values in both the MRS and the LAPTg broths. Conclusions: The optimal growth conditions were recorded in MRS broth, with an initial pH of 6.5 and a temperature of 37°C. The maximum bacteriocin activity was obtained in LAPTg after 6 h at 37°C, and at an initial pH of 6.5 or 8.0. Significance and Impact of the Study: The application of a complete factorial design, and the evaluation of the growth parameters through the Gompertz model, enabled a rapid and simultaneous exploration of the influence of pH, temperature and growth medium on both growth and bacteriocin production by vaginal Lact. salivarius CRL 1328.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bacteriocin Production
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Vaginal Lactobacillus
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Factorial Design
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Gompertz Model
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Influence of pH, temperature and culture media on the growth and bacteriocin production by vaginal Lactobacillus salivarius CRL 1328
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-23T18:13:18Z
dc.identifier.eissn
1365-2672
dc.journal.volume
93
dc.journal.number
4
dc.journal.pagination
714-724
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Bru Chauve, Elena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Wiese, Birgitt. Hannover Medical School. Institute of Biometry; Alemania
dc.description.fil
Fil: Pesce de Ruiz Holgado, Aida Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.journal.title
Journal of Applied Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1046/j.1365-2672.2002.01753.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2672.2002.01753.x
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