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dc.contributor.author
Fernández, Jorge Gastón
dc.contributor.author
Fernández Baldo, Martín Alejandro
dc.contributor.author
Muñoz, Claudio Javier
dc.contributor.author
Salinas, Eloy
dc.contributor.author
Raba, Julio
dc.contributor.author
Sanz Ferramola, Maria Isabel
dc.date.available
2016-05-24T19:37:49Z
dc.date.issued
2014-02
dc.identifier.citation
Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Muñoz, Claudio Javier; Salinas, Eloy; Raba, Julio; et al.; Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples; Hainan Medical University; Journal of Coastal Life Medicine; 2; 2; 2-2014; 125-131
dc.identifier.issn
2309-6152
dc.identifier.uri
http://hdl.handle.net/11336/5828
dc.description.abstract
Detection of Botrytis cinerea latent infections on apples before storage is essential for effective control strategies in the fruit postharvest industry. In the present study a PCR detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer (IGS) region as internal control were utilized to reveal the presence of symptomless infections on apple fruits. The method molecular proved to be highly specific and sensitive in detecting latent infections, revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40 and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper The results obtained of DNA quantification method were compared with ELISA assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible control appropriate strategies. Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Hainan Medical University
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Botrytis Cinerea
dc.subject
Latent Infection
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Transposable Elements
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Symptomless Apples
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Micología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-05-16T20:17:53Z
dc.identifier.eissn
2309-5288
dc.journal.volume
2
dc.journal.number
2
dc.journal.pagination
125-131
dc.journal.pais
China
dc.journal.ciudad
Haikou
dc.description.fil
Fil: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
dc.description.fil
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Muñoz, Claudio Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza - San Juan. Estación Experimental Agropecuaria Mendoza; Argentina
dc.description.fil
Fil: Salinas, Eloy. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
dc.journal.title
Journal of Coastal Life Medicine
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/ark/http://www.jclmm.com/qk/20142/9.pdf
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/10.12980/JCLM.2.2014JCLM-2013-0005
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.12980/JCLM.2.2014JCLM-2013-0005
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