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dc.contributor.author
Carletti, Tamara
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Barreto, Carmo
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Mesplet, Maria
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Mira, Anabela
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Weir, William
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Shiels, Brian
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Gonzalez Oliva, Abel
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Schnittger, Leonhard
dc.contributor.author
Jacobsen, Monica Ofelia
dc.date.available
2018-09-04T16:40:23Z
dc.date.issued
2016-02
dc.identifier.citation
Carletti, Tamara; Barreto, Carmo; Mesplet, Maria; Mira, Anabela; Weir, William; et al.; Characterization of a papain-like cysteine protease essential for the survival of Babesia ovis merozoites; Elsevier Gmbh; Ticks and Tick-borne Diseases; 7; 1; 2-2016; 85-93
dc.identifier.issn
1877-9603
dc.identifier.uri
http://hdl.handle.net/11336/58246
dc.description.abstract
Babesia ovis, a tick-transmitted intraerythrocytic protozoan parasite, causes severe infections in small ruminants from Southern Europe, Middle East, and Northern Africa. With the aim of finding potential targets for the development of control methods against this parasite, sequence analysis of its genome led to the identification of four putative cysteine proteases of the C1A family. Orthology between B. ovis, B. bovis, T. annulata, and T. parva sequences showed that each B. ovis C1A peptidase sequence clustered within one of the four ortholog groups previously reported for these piroplasmids. The ortholog of bovipain-2 of B. bovis and falcipain-2 of Plasmodium falciparum, respectively, was designated "ovipain-2" and further characterized. In silico analysis showed that ovipain-2 has the typical topology of papain-like cysteine peptidases and a highly similar predicted three dimensional structure to bovipain-2 and falcipain-2, suggesting susceptibility to similar inhibitors. Immunoblotting using antibodies raised against a recombinant form of ovipain-2 (r-ovipain-2) demonstrated expression of ovipain-2 in in vitro cultured B. ovis merozoites. By immunofluorescence, these antibodies reacted with merozoites and stained the cytoplasm of infected erythrocytes. This suggests that ovipain-2 is secreted by the parasite and could be involved in intra- and extracellular digestion of hemoglobin and/or cleavage of erythrocyte proteins facilitating parasite egress. A significant reduction in the percentage of parasitized erythrocytes was obtained upon incubation of B. ovis in vitro cultures with anti-r-ovipain-2 antibodies, indicating an important functional role for ovipain-2 in the intra erythrocytic development cycle of this parasite. Finally, studies of the reactivity of sera from B. ovis-positive and negative sheep against r-ovipain-2 showed that this protease is expressed in vivo, and can be recognized by host antibodies. The results of this study suggest that ovipain-2 constitutes a potential target for immunotherapies and drug development against ovine babesiosis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Gmbh
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Babesia Ovis
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Cysteine Proteases
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Drug Development
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In Vitro Neutralization
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Ovine Babesiosis
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Ovipain-2
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Subunit Vaccine
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Characterization of a papain-like cysteine protease essential for the survival of Babesia ovis merozoites
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-04T15:11:53Z
dc.journal.volume
7
dc.journal.number
1
dc.journal.pagination
85-93
dc.journal.pais
Alemania
dc.description.fil
Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
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Fil: Barreto, Carmo. Universidade Nova; Portugal. Instituto de Biologia Experimental e Tecnologica; Portugal
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Fil: Mesplet, Maria. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Weir, William. University of Glasgow; Reino Unido
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Fil: Shiels, Brian. University of Glasgow; Reino Unido
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Fil: Gonzalez Oliva, Abel. Universidade Nova; Portugal. Instituto de Biologia Experimental e Tecnologica; Portugal
dc.description.fil
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Ticks and Tick-borne Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ttbdis.2015.09.002
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1877959X1530008X
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