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dc.contributor.author
Tribelli, Paula Maria
dc.contributor.author
Hay, Anthony
dc.contributor.author
Lopez, Nancy Irene
dc.date.available
2015-05-27T15:47:44Z
dc.date.issued
2013-10-16
dc.identifier.citation
Tribelli, Paula Maria; Hay, Anthony; Lopez, Nancy Irene; The global anaerobic regulator Anr, is involved in cell attachment and aggregation influencing the first stages of biofilm development in Pseudomonas extremaustralis; Public Library Science; Plos One; 8; 10; 16-10-2013; 1-8;
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/577
dc.description.abstract
Pseudomonas extremaustralis is a versatile Antarctic bacterium, able to grow under microaerobic and anaerobic conditions and is related to several non-pathogenic Pseudomonads. Here we report on the role of the global anaerobic regulator Anr, in the early steps of P. extremaustralis biofilm development. We found that the anr mutant was reduced in its ability to attach, to form aggregates and to display twitching motility but presented higher swimming motility than the wild type. In addition, microscopy revealed that the wild type biofilm contained more biomass and was thicker, but were less rough than that of the anr mutant. In silico analysis of the P. extremaustralis genome for Anr-like binding sites led to the identification of two biofilm-related genes as potential targets of this regulator. When measured using Quantitative Real Time PCR, we found that the anr mutant expressed lower levels of pilG , which encodes a component of Type IV pili and has been previously implicated in cellular adhesion. Levels of morA, involved in signal transduction and flagella development, were also lower in the mutant. Our data suggest that under low oxygen conditions, such as those encountered in biofilms, Anr differentially regulates aggregation and motility thus affecting the first stages of biofilm formation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Biofilm
dc.subject
Pseudomonas
dc.subject
Anr
dc.subject
Cell Attachment
dc.subject.classification
Ciencias Naturales y Exactas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
Biología Celular, Microbiología
dc.title
The global anaerobic regulator Anr, is involved in cell attachment and aggregation influencing the first stages of biofilm development in Pseudomonas extremaustralis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
10
dc.journal.pagination
1-8
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Tribelli, Paula Maria. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Quimica Biologica de la Facultad de Cs. Exactas y Naturales;
dc.description.fil
Fil: Hay, Anthony.
dc.description.fil
Fil: Lopez, Nancy Irene. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Quimica Biologica de la Facultad de Cs. Exactas y Naturales;
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0076685
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org
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