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dc.contributor.author
Foresti, María Laura
dc.contributor.author
Errazu, Alberto Felipe
dc.contributor.author
Ferreira, María Luján
dc.date.available
2018-08-30T20:00:09Z
dc.date.issued
2005-08
dc.identifier.citation
Foresti, María Laura; Errazu, Alberto Felipe; Ferreira, María Luján; Effect of several reaction parameters in the solvent-free ethyl oleate synthesis using Candida rugosa lipase immobilised on polypropylene; Elsevier Science Sa; Biochemical Engineering Journal; 25; 1; 8-2005; 69-77
dc.identifier.issn
1369-703X
dc.identifier.uri
http://hdl.handle.net/11336/57777
dc.description.abstract
Lipase from Candida rugosa was immobilised onto polypropylene powder by physical adsorption. The immobilised catalyst (CR/PP) was used in the enzymatic synthesis of ethyl oleate in solvent-free medium. The influence of the initial water content, acidity of the aqueous media added, mass of catalyst, reaction temperature, substrate ratio, etc., on enzymatic activity, has been studied. Comparison of specific enzymatic activities achieved using the prepared catalyst and the crude lipase demonstrated that C. rugosa lipase was interfacially activated upon its adsorption on polypropylene. Besides, immobilisation of the lipase led to enhanced thermal stability. Experimental data reported in this manuscript do not belong to equilibrium data but to enzymatic activity attained in the first 2 h of reaction. After this period, it was shown that, in the current synthesis, deactivation/agglomeration/inhibition of the catalyst prevented further CRL activity. However, 2 h measurements allowed fulfilling the aim of this work: the determination of the best conditions for ethyl oleate production in short periods of time, using an immobilised derivative of a relatively cheap lipase as it is C. rugosa lipase. Best results were achieved in the reaction performed at 45°C and 350 rpm, with an initial stoichiometric ratio of substrates, 20% of aqueous content, and mediated by 50 mg of CR/PP (0.0585 mmol/mg of CR-h). The deleterious effect of ethanol excess and agglomeration of the native and immobilised catalyst have been analysed. © 2005 Elsevier B.V. All rights reserved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Sa
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Aggregation
dc.subject
Ethyl Oleate Synthesis
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Inhibiting Effect
dc.subject
Lipase
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Reaction Parameters
dc.subject.classification
Biotecnología Industrial
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Effect of several reaction parameters in the solvent-free ethyl oleate synthesis using Candida rugosa lipase immobilised on polypropylene
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-21T13:44:56Z
dc.journal.volume
25
dc.journal.number
1
dc.journal.pagination
69-77
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Foresti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
dc.description.fil
Fil: Errazu, Alberto Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
dc.description.fil
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
dc.journal.title
Biochemical Engineering Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bej.2005.04.002
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1369703X05000860


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