An Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for Pseudomonas aeruginosa Biofilm Eradication

Abstract

Biofilms cause biofouling, pipe plugging, prostheses colonization, disease, and nosocomial infections. Bacterial biofilms are more resilient to sterilization methods than planktonic bacteria; therefore, better control methods are required. The use of gas discharge plasmas is an appropriate alternative since plasmas contain a mixture of reactive agents well-known for bacterial decontamination.This study assessed culture medium-abiotic surface combinations leading to robust biofilms; and tested an air-based coaxial dielectric barrier discharge (DBD) plasma source on Pseudomonas aeruginosa biofilms grown in continuous culture under those selected conditions. Biofilms were eradicated after a 15-minutes plasma treatment resulting in a CFU/mL decrease of 5.6 log10 units. CFU/mL decreases of 1.6 and 2.7 log10 units were achieved after a 3-min plasma exposure to ambient and moistened air plasma respectively although viability assays showed that some cells were alive. Moistened-air plasma resulted in a faster biofilm inactivation with decimal reduction times of 1.14 and 4.36 minutes. The coaxial DBD air-based plasma source presented here is effective for Pseudomonas biofilm inactivation; affordable since it does not rely on expensive gases; and easy to handle for indirect surface treatment. The search for the best combination medium-surface leading to robust biofilms prior to plasma treatment was not previously assessed.