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dc.contributor.author
Garcia, Daniel Asmed
dc.contributor.author
Vendrell, Iolanda
dc.contributor.author
Galofré, Mireia
dc.contributor.author
Suñol, Cristina
dc.date.available
2018-08-29T20:06:29Z
dc.date.issued
2008-12
dc.identifier.citation
Garcia, Daniel Asmed; Vendrell, Iolanda; Galofré, Mireia; Suñol, Cristina; GABA released from cultured cortical neurons influences the modulation of t-[35S]butylbicyclophosphorothionate binding at the GABAA receptor: Effects of thymol; Elsevier Science; European Journal of Pharmacology; 600; 1-3; 12-2008; 26-31
dc.identifier.issn
0014-2999
dc.identifier.uri
http://hdl.handle.net/11336/57583
dc.description.abstract
Thymol is a monoterpene that specifically interacts with synaptic neural functions in neuronal GABA-operated Cl- channels. Here we explore the effects of thymol, and propofol as positive control, on t-[35S]butylbicyclophosphorothionate ([35S]TBPS) binding in primary cultures of cortical neurons. The study includes a meaningful analysis of the effect of various exposure buffers, and their correlation with GABA released from cells, chloride influx through the GABAA receptor and GABA transporter activity. Cell viability was also determined. Thymol and propofol inhibited the binding of [35S]TBPS to cells exposed to Tris-citrate-NaCl buffer whereas a biphasic effect was observed in HEPES solution. The different effects of the two buffers analysed are due to the higher capacity of Tris-citrate-NaCl buffer to induce the release of endogenous GABA facilitating the binding of [35S]TBPS to its recognition site at the GABAA receptor. Released GABA in the presence of this buffer was inhibited by the neuronal GABA transporter inhibitor SKF 100330-A. Tris-citrate-NaCl buffer also induced a chloride influx, which was reverted by picrotoxinin. TBPS binding in living cells is facilitated by GABA released from the cells, which in turn activates basal GABAA receptor activity. The results deepen on the allosteric mechanism of thymol as positive modulator of the GABAA receptor. Furthermore, we corroborate [35S]TBPS binding as an important test to verify the capacity of drugs to act on and recognize a site at the GABAA receptor.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
[35s]Tbps Binding
dc.subject
Buffer Effect, Neuronal Gaba Transporter
dc.subject
Chloride Uptake
dc.subject
Gaba Release
dc.subject
Gabaa Receptor
dc.subject
Thymol
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
GABA released from cultured cortical neurons influences the modulation of t-[35S]butylbicyclophosphorothionate binding at the GABAA receptor: Effects of thymol
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-13T17:06:21Z
dc.journal.volume
600
dc.journal.number
1-3
dc.journal.pagination
26-31
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; Argentina. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Vendrell, Iolanda. Consejo Superior de Investigaciones Científicas; España. Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública; España
dc.description.fil
Fil: Galofré, Mireia. Consejo Superior de Investigaciones Científicas; España. Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública; España
dc.description.fil
Fil: Suñol, Cristina. Consejo Superior de Investigaciones Científicas; España. Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública; España
dc.journal.title
European Journal of Pharmacology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014299908010303
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ejphar.2008.10.013


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