Artículo
Extrinsic functions of lectin domains in O-N-acetylgalactosamine glycan biosynthesis
Lorenz, Virginia
; Ditamo, Yanina
; Cejas, Romina Beatríz
; Carrizo Garcia, Maria Elena
; Bennett, Eric P.; Clausen, Henrik; Nores, Gustavo Alejandro
; Irazoqui, Fernando Jose
Fecha de publicación:
12/2016
Editorial:
American Society for Biochemistry and Molecular Biology
Revista:
Journal of Biological Chemistry (online)
ISSN:
0021-9258
e-ISSN:
1083-351X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Glycan biosynthesis occurs mainly in Golgi. Molecular organization and functional regulation of this process are not well understood. We evaluated the extrinsic effect of lectin domains (β-trefoil fold) of polypeptide GalNAc-transferases (ppGalNAc-Ts) on catalytic activity of glycosyltransferases during O-GalNAc glycan biosynthesis. The presence of lectin domain T3lec or T4lec during ppGalNAc-T2 and ppGalNAc-T3 catalytic reaction had a clear inhibitory effect on GalNAc-T activity. Interaction of T3lec or T4lec with ppGalNAc-T2 catalytic domain was not mediated by carbohydrate. T3lec, but not T2lec and T4lec, had a clear activating effect on Drosophila melanogaster core 1 galactosyltransferase enzyme activity and a predominant inhibitory effect on in vivo human core 1 glycan biosynthesis. The regulatory role of the β-trefoil fold of ppGalNAc-Ts in enzymatic activity of glycosyltransferases involved in the O-glycan biosynthesis pathway, described here for the first time, helps clarify the mechanism of biosynthesis of complex biopolymers (such as glycans) that is not template-driven.
Palabras clave:
Glycobiology
,
Glycoprotein Biosynthesis
,
Glycosylation Inhibitor
,
Lectin
,
Mucin
Archivos asociados
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Colecciones
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Lorenz, Virginia; Ditamo, Yanina; Cejas, Romina Beatríz; Carrizo Garcia, Maria Elena; Bennett, Eric P.; et al.; Extrinsic functions of lectin domains in O-N-acetylgalactosamine glycan biosynthesis; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 291; 49; 12-2016; 25339-25350
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