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dc.contributor.author
Savoy, Graciela
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dc.contributor.author
Foucaud-Scheunemann, Catherine
dc.contributor.author
Ferchichi, Mohamed
dc.contributor.author
Hemme, Denis
dc.date.available
2018-08-27T19:29:25Z
dc.date.issued
2002-10-17
dc.identifier.citation
Savoy, Graciela; Foucaud-Scheunemann, Catherine; Ferchichi, Mohamed; Hemme, Denis; Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 35; 5; 17-10-2002; 428-432
dc.identifier.issn
0266-8254
dc.identifier.uri
http://hdl.handle.net/11336/57275
dc.description.abstract
Aims: To demonstrate the mechanism of glutamate uptake in the dairy strain Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208, and to characterize key aspects of the system. Methods and Results: Glutamate uptake proceeded via an active transport system requiring an exogenous source of energy. The system also transported aspartate and glutamine. It was unique, with a Kt of 2.8 μmoll-1 and a Vmax of 900 μmol s-1 (g dry weight)-1. The activity was optimal at pH 7.3 and 50°C, was independent of the glutamate charge, and was enhanced by Mn2+ + Mg2+ in combination. Inhibition of the activity by uncouplers and ionophores showed that transport was driven by an ATP-dependent mechanism involving the proton-motive force. This inhibition was partially abolished in the presence of both Mn2+ and Mg2+. Conclusions: We demonstrated for the first time that an active transport system governs the uptake of the essential amino acid glutamate in Lact. delbrueckii subsp. bulgaricus CNRZ 208, the activity of which is enhanced by a combination of Mn2+ and Mg2+. Significance and Impact of the Study: The potential of the findings is discussed with reference to the growth of Lact. delbrueckii subsp. bulgaricus in mixed-strain cultures for the dairy industry.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Glutamate Uptake
dc.subject
Lactobacillus Delbrueckii Subsp. Bulgaricus
dc.subject
Amino Acid Transport
dc.title
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-23T18:11:58Z
dc.identifier.eissn
1472-765X
dc.journal.volume
35
dc.journal.number
5
dc.journal.pagination
428-432
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Savoy, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Foucaud-Scheunemann, Catherine. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia
dc.description.fil
Fil: Ferchichi, Mohamed. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia
dc.description.fil
Fil: Hemme, Denis. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia
dc.journal.title
Letters in Applied Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1046/j.1472-765X.2002.01208.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1472-765X.2002.01208.x
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