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dc.contributor.author
Raya, Raul Ricardo
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dc.contributor.author
Oot, Rebecca A.
dc.contributor.author
Moore Maley, Ben
dc.contributor.author
Wieland, Serena
dc.contributor.author
Callaway, Todd R.
dc.contributor.author
Kutter, Elizabeth M.
dc.contributor.author
Brabban, Andrew D.
dc.date.available
2018-08-27T18:39:57Z
dc.date.issued
2014-10-01
dc.identifier.citation
Raya, Raul Ricardo; Oot, Rebecca A.; Moore Maley, Ben; Wieland, Serena; Callaway, Todd R.; et al.; Naturally resident and exogenously applied T4-like and T5-like bacteriophages can reduce Escherichia coli O157; Taylor & Francis; Bacteriophage; 1; 1; 1-10-2014; 15-24
dc.identifier.issn
2159-7073
dc.identifier.uri
http://hdl.handle.net/11336/57254
dc.description.abstract
In preparing sheep for an in vivo Escherichia coli O157:H7 eradication trial, we found that 20/39 members of a single flock were naturally colonized by O157:H7-infecting phages. Characterization showed these were all one phage type (subsequently named CEV2) infecting 15/16 O157:H7, 7/72 ECOR, and common lab strains. Further characterization by PFGE (genome~120kb), restriction enzyme digest (DNA appears unmodified), receptor studies (FhuA but not TonB is required for infection) and sequencing (>95% nucleotide identity) showed it is a close relative of the classically studied coliphage T5. Unlike T5, CEV2 infects O157:H7 in vitro, both aerobically and anaerobically, rapidly adsorbing and killing, but resistant mutants regrew within 24 h. When used together with T4-like CEV1 (MOI~2 per phage), bacterial killing was longer lasting. CEV2 did not reproduce when co-infecting the same cell as CEV1, presumably succumbing to CEV1's ability to shut off transcription of cytosine-containing DNA. In vivo sheep trials to remove resident O157:H7 showed that a cocktail of CEV2 and CEV1 (~1011 total pfu) applied once orally was more effective (>99.9% reduction) than CEV1 alone (~99%) compared to the untreated phage-free control. Those sheep naturally carrying CEV2, receiving no additional phage treatment, had the lowest O157:H7 levels (~99.99% reduction). These data suggest that phage cocktails are more effective than individual phage in removing O157:H7 that have taken residence if the phage work in concert with one another and that naturally resident O157:H7-infecting phages may prevent O157:H7 gut colonization and be one explanation for the transient O157:H7 colonization in ruminants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bacteriophage
dc.subject
Therapy
dc.subject
Escherichia Coli O157:H7
dc.subject
Food Safety
dc.subject
Food-Borne Pathogen
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Naturally resident and exogenously applied T4-like and T5-like bacteriophages can reduce Escherichia coli O157
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-10T14:05:09Z
dc.identifier.eissn
2159-7081
dc.journal.volume
1
dc.journal.number
1
dc.journal.pagination
15-24
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Philadelphia, PA
dc.description.fil
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. The Evergreen State College; Estados Unidos
dc.description.fil
Fil: Oot, Rebecca A.. The Evergreen State College; Estados Unidos
dc.description.fil
Fil: Moore Maley, Ben. The Evergreen State College; Estados Unidos
dc.description.fil
Fil: Wieland, Serena. The Evergreen State College; Estados Unidos
dc.description.fil
Fil: Callaway, Todd R.. Food and Feed Safety Research Unit. Agricultural Research Service; Estados Unidos
dc.description.fil
Fil: Kutter, Elizabeth M.. The Evergreen State College; Estados Unidos
dc.description.fil
Fil: Brabban, Andrew D.. The Evergreen State College; Estados Unidos
dc.journal.title
Bacteriophage
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4161/bact.1.1.14175
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.4161/bact.1.1.14175
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