Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant

Abstract

Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants of Achyrocline satureioides. Organogenesis was induced from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 30g·L-1) containing different combinations of 6-benzyladenine (BA) and alpha-naphtalenacetic acid (NAA) under 116 micromol·m-2·s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 27±2ºC. The regeneration was similar for every tested explant and varied between 64 and 83%. The number of buds formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In order to stimulate In vitro rooting, regenerative leaves were sub cultured from the best induction medium in MS lacking plant growth regulators for the same periods. Every plantlet raised In vitro was phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was established.