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Artículo

Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.

Felici, Carlos EmilianoIcon ; Molina, Alicia; Almeida, César AméricoIcon ; Fernández Baldo, Martín AlejandroIcon ; Zirulnik, Fanny; Gomez, Maria Roxana AnabelIcon
Fecha de publicación: 06/2014
Editorial: Hainan Medical University
Revista: Journal of Coastal Life Medicine
ISSN: 2309-6152
e-ISSN: 2309-5288
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Analítica

Resumen

Objective: To analyze the effect of Cadmium (Cd) on the metabolism of reduced glutathione (GSH) in roots and leaves of Glycine max L. Methods: A capillary electrophoresis methodology was optimized to determinate simultaneously GSH and reduced and oxidized GSH in a precise and accurate way. The functional role of the genes involved in GSH cellular metabolism (γ-glutamylcysteine synthetase), GSH synthetase and glutathione reductase (GR) was evaluated. Finally, the activities of antioxidant enzymes as GR and superoxide dismutases were determinate. Results: The studies of γ-glutamylcysteine synthetase and GSH synthetase gene expression showed an increase and GR showed a decrease in Cd-treated plants. GR and superoxide dismutases activities increased at 24 h and 6 h respectively in roots under Cd exposure. GSH content was higher and showed a significant increase in roots and leaves at 6 h and 24 h of treatment. Conclusions: The results of the present study showed a better understanding in signaling pathway by alterations in antioxidant mechanisms by Cd in soybean seedlings.
Palabras clave: Soybean , Cadmium Toxicity , Oxidative Stress , Antioxidant Defense , Glutathione , Capillary Electrophoresis
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/5707
DOI: http://dx.doi.org/10.12980/JCLM.2.2014JCLM-2014-0077
URL: http://www.jclmm.com/qk/201410/6.pdf
Colecciones
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Felici, Carlos Emiliano; Molina, Alicia; Almeida, César Américo; Fernández Baldo, Martín Alejandro; Zirulnik, Fanny; et al.; Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.; Hainan Medical University; Journal of Coastal Life Medicine; 2; 10; 6-2014; 791-798
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