Artículo
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle
Campero, Lucía María
; Moreno Gonzalo, Javier; Venturini, María Cecilia; Moré, Gastón Andrés
; Dellarupe, Andrea
; Rambeaud, Magdalena
; Echaide, Ignacio Eduardo; Valentini, Aurora Beatriz; Campero, Lucía María
; Moore, Dadin Prando
; Cano, Dora B.; Fort, Marcelo; Mota, Rinaldo A.; Serrano Martínez, Marcos E.; Cruz-Vázquez, Carlos; Ortega Mora, Luis M.; Álvarez García, Gema
Fecha de publicación:
01/2018
Editorial:
Springer
Revista:
Tropical Animal Health And Production
ISSN:
0049-4747
e-ISSN:
1573-7438
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2.
Palabras clave:
Bovine Neosporosis
,
Ibero-America
,
Serological Assay Trial
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Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Campero, Lucía María; Moreno Gonzalo, Javier; Venturini, María Cecilia; Moré, Gastón Andrés; Dellarupe, Andrea; et al.; An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle; Springer; Tropical Animal Health And Production; 50; 1; 1-2018; 75-84
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