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dc.contributor.author
Pérez Sirkin, Daniela Irina
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dc.contributor.author
Lafont, Anne Gaëlle
dc.contributor.author
Kamech, Nédia
dc.contributor.author
Somoza, Gustavo Manuel
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dc.contributor.author
Vissio, Paula Gabriela
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dc.contributor.author
Dufour, Sylvie
dc.date.available
2018-08-22T16:30:22Z
dc.date.issued
2017-08
dc.identifier.citation
Pérez Sirkin, Daniela Irina; Lafont, Anne Gaëlle; Kamech, Nédia; Somoza, Gustavo Manuel; Vissio, Paula Gabriela; et al.; Conservation of three-dimensional helix-loop-helix structure through the vertebrate lineage reopens the cold case of gonadotropin-releasing hormone-associated Peptide; Frontiers Research Foundation; Frontiers in Endocrinology; 8; AUG; 8-2017; 1-12
dc.identifier.issn
1664-2392
dc.identifier.uri
http://hdl.handle.net/11336/56558
dc.description.abstract
GnRH-associated peptide (GAP) is the C-terminal portion of the gonadotropin-releasing hormone (GnRH) preprohormone. Although it was reported in mammals that GAP may act as a prolactin-inhibiting factor and can be co-secreted with GnRH into the hypophyseal portal blood, GAP has been practically out of the research circuit for about 20 years. Comparative studies highlighted the low conservation of GAP primary amino acid sequences among vertebrates, contributing to consider that this peptide only participates in the folding or carrying process of GnRH. Considering that the three-dimensional (3D) structure of a protein may define its function, the aim of this study was to evaluate if GAP sequences and 3D structures are conserved in the vertebrate lineage. GAP sequences from various vertebrates were retrieved from databases. Analysis of primary amino acid sequence identity and similarity, molecular phylogeny, and prediction of 3D structures were performed. Amino acid sequence comparison and phylogeny analyses confirmed the large variation of GAP sequences throughout vertebrate radiation. In contrast, prediction of the 3D structure revealed a striking conservation of the 3D structure of GAP1 (GAP associated with the hypophysiotropic type 1 GnRH), despite low amino acid sequence conservation. This GAP1 peptide presented a typical helix-loop-helix (HLH) structure in all the vertebrate species analyzed. This HLH structure could also be predicted for GAP2 in some but not all vertebrate species and in none of the GAP3 analyzed. These results allowed us to infer that selective pressures have maintained GAP1 HLH structure throughout the vertebrate lineage. The conservation of the HLH motif, known to confer biological activity to various proteins, suggests that GAP1 peptides may exert some hypophysiotropic biological functions across vertebrate radiation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Research Foundation
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Evolution
dc.subject
Gnrh-Associated Peptide
dc.subject
Helix-Loop-Helix
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Phylogeny
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Protein 3d Structure
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Teleosts
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Vertebrates
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Conservation of three-dimensional helix-loop-helix structure through the vertebrate lineage reopens the cold case of gonadotropin-releasing hormone-associated Peptide
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-22T12:54:24Z
dc.journal.volume
8
dc.journal.number
AUG
dc.journal.pagination
1-12
dc.journal.pais
Suiza
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dc.description.fil
Fil: Pérez Sirkin, Daniela Irina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
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Fil: Lafont, Anne Gaëlle. Museum National d'Histoire Naturelle; Francia
dc.description.fil
Fil: Kamech, Nédia. Museum National d'Histoire Naturelle; Francia
dc.description.fil
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
dc.description.fil
Fil: Vissio, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
dc.description.fil
Fil: Dufour, Sylvie. Museum National d'Histoire Naturelle; Francia
dc.journal.title
Frontiers in Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fendo.2017.00207
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fendo.2017.00207/full
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