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dc.contributor.author
Van der Meulen, Roel
dc.contributor.author
Grosu Tudor, Silvia
dc.contributor.author
Mozzi, Fernanda Beatriz
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dc.contributor.author
Vaningelgem, Frederik
dc.contributor.author
Zamfir, Medana
dc.contributor.author
Font, Graciela Maria
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dc.contributor.author
De Vuyst, Luc
dc.date.available
2018-08-21T21:15:53Z
dc.date.issued
2007-09-30
dc.identifier.citation
Van der Meulen, Roel; Grosu Tudor, Silvia; Mozzi, Fernanda Beatriz; Vaningelgem, Frederik; Zamfir, Medana; et al.; Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved; Elsevier Science; International Journal of Food Microbiology; 118; 3; 30-9-2007; 250-258
dc.identifier.issn
0168-1605
dc.identifier.uri
http://hdl.handle.net/11336/56482
dc.description.abstract
A total of 174 lactic acid bacteria (LAB) strains isolated from dairy and cereal products were screened for the production of exopolysaccharides (EPS). Therefore, a rapid screening method was developed based on ultrafiltration and gel permeation chromatography. Furthermore, a screening through the polymerase chain reaction (PCR) was performed with primer pairs targeting different genes involved in EPS production. Nine isolates produced a homopolysaccharide of the glucan type, whereas only one strain produced a heteropolysaccharide. The production of a glucan by a strain of Lactococcus lactis and the production of a heteropolysaccharide by a strain of Lactobacillus curvatus are reported for the first time. The PCR screening revealed many positive strains. For three of the ten EPS-producing strains, no corresponding genes could be detected. Furthermore, a lot of strains possessed one or more eps genes but did not produce an EPS. Therefore, a screening on the molecular level should always be accompanied by another screening method that is able to distinguish true EPS producer strains from non-producing ones. Statistical analysis did not reveal any relationship between the type and origin of the strains, the presence or absence of a capsular polysaccharide or EPS, and the presence or absence of eps genes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Exopolysaccharides
dc.subject
Lactic Acid Bacteria
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Screening
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Ultrafiltration
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-23T18:13:47Z
dc.identifier.eissn
1879-3460
dc.journal.volume
118
dc.journal.number
3
dc.journal.pagination
250-258
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Van der Meulen, Roel. Vrije Universiteit Brussel; Bélgica
dc.description.fil
Fil: Grosu Tudor, Silvia. Vrije Universiteit Brussel; Bélgica
dc.description.fil
Fil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Vaningelgem, Frederik. Vrije Universiteit Brussel; Bélgica
dc.description.fil
Fil: Zamfir, Medana. Vrije Universiteit Brussel; Bélgica. Romanian Academy. Cell Biology Department. Institute of Biology; Rumania
dc.description.fil
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: De Vuyst, Luc. Vrije Universiteit Brussel; Bélgica
dc.journal.title
International Journal of Food Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ijfoodmicro.2007.07.014
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160507003601
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