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dc.contributor.author
Castellano, Patricia Haydee  
dc.contributor.author
Vignolo, Graciela Margarita  
dc.contributor.author
Farias, Ricardo Norberto  
dc.contributor.author
Arrondo Jose Luis  
dc.contributor.author
Chehin, Rosana Nieves  
dc.date.available
2018-08-21T17:42:36Z  
dc.date.issued
2007-01-20  
dc.identifier.citation
Castellano, Patricia Haydee; Vignolo, Graciela Margarita; Farias, Ricardo Norberto; Arrondo Jose Luis; Chehin, Rosana Nieves; Molecular view by Fourier transform infrared spectroscopy of the relationship between lactocin 705 and membranes: Speculations on antimicrobial mechanism; American Society for Microbiology; Applied And Environmental Microbiology; 73; 2; 20-1-2007; 415-420  
dc.identifier.issn
0099-2240  
dc.identifier.uri
http://hdl.handle.net/11336/56340  
dc.description.abstract
Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705α and Lac705β. Neither Lac705α nor Lac705β displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism of action, Fourier transform infrared spectroscopy was used to investigate the interactions of each peptide (Lac705α and Lac705β) with dipalmitoylphosphatidylcholine liposomal membranes. Both peptides show the ability to interact with the zwitterionic membrane but at different bilayer levels. While Lac705α interacts with the interfacial region inducing dehydration, Lac705β peptide interacts with only the hydrophobic core. This paper presents the first experimental evidence that supports the hypothesis that Lac705α and Lac705β peptides could form a transmembrane oligomer. From the obtained results, a mechanism of action of lactocin 705 on membrane systems is proposed. The component Lac705α could induce the dehydration of the bilayer interfacial region, and the Lac705β peptide could insert in the hydrophobic region of the membrane where the peptide has adequate conditions to achieve the oligomerization.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Society for Microbiology  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Antimicrobial Peptide  
dc.subject
Lactocin 705  
dc.subject
Mechanism of Action  
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Ftir  
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Fourier Transform Infrared Spectroscopy  
dc.subject.classification
Biología Celular, Microbiología  
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Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Molecular view by Fourier transform infrared spectroscopy of the relationship between lactocin 705 and membranes: Speculations on antimicrobial mechanism  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-07-23T18:11:06Z  
dc.identifier.eissn
1098-5336  
dc.journal.volume
73  
dc.journal.number
2  
dc.journal.pagination
415-420  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Washington  
dc.description.fil
Fil: Castellano, Patricia Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina  
dc.description.fil
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina  
dc.description.fil
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Arrondo Jose Luis. Universidad Politécnica de Valencia; España  
dc.description.fil
Fil: Chehin, Rosana Nieves. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina  
dc.journal.title
Applied And Environmental Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/AEM.01293-06  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/73/2/415