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dc.contributor.author
Foresti, María Laura
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dc.contributor.author
Ferreira, María Luján
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dc.date.available
2018-08-17T20:22:33Z
dc.date.issued
2007-03
dc.identifier.citation
Foresti, María Laura; Ferreira, María Luján; Chitosan-immobilized lipases for the catalysis of fatty acid esterifications; Elsevier Science Inc; Enzyme and Microbial Technology; 40; 4; 3-2007; 769-777
dc.identifier.issn
0141-0229
dc.identifier.uri
http://hdl.handle.net/11336/56272
dc.description.abstract
Lipases from Candida rugosa, Pseudomonas fluorescens and Candida antarctica B were immobilized onto chitosan and glutaraldehyde-pretreated chitosan powders. The prepared biocatalysts were assayed in the direct esterification of oleic acid and ethanol to produce the ethyl oleate. In order to maximize ester production and avoid solvent-related costs (the solvent itself, solvent recovery and solvent recycle), the synthesis of ethyl oleate was performed in a solvent-free system. The different structures of the lipases chosen for the catalysis led to very different activity levels, with C. antarctica B derivatives being the most active ones. The parametric study performed revealed that the best operation conditions for ester synthesis are found at mild temperatures (35-45 °C), and in "biphasic systems" (two liquid phases), generated upon addition of relatively high quantities of water to the mixture of substrates. The reduction of the concentration of water in the organic reactive phase of biphasic systems favored ester synthesis, with higher ester yields in the first hours of reaction than those measured in systems with no added water. In the optimum conditions mentioned the biocatalyst resulting from the immobilization of lipase from C. antarctica B onto untreated chitosan powder led to 75% conversion of the fatty acid in 24 h of reaction. The stability of this catalyst also proved to be very attractive with five consecutive 24 h uses with a residual activity of 90-95%.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Chitosan
dc.subject
Esterification
dc.subject
Immobilization
dc.subject
Lipase
dc.subject.classification
Otras Ingeniería Química
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dc.subject.classification
Ingeniería Química
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Chitosan-immobilized lipases for the catalysis of fatty acid esterifications
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-08T16:38:49Z
dc.journal.volume
40
dc.journal.number
4
dc.journal.pagination
769-777
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Foresti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
dc.description.fil
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
dc.journal.title
Enzyme and Microbial Technology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.enzmictec.2006.06.009
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S014102290600336X
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