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dc.contributor.author
Arena, Miriam Elisabet
dc.contributor.author
Martínez Pastur, Guillermo José
dc.contributor.author
Benavides, Maria Patricia
dc.contributor.author
Curvetto, Nestor Raul
dc.date.available
2018-08-15T21:23:16Z
dc.date.issued
2005-12
dc.identifier.citation
Arena, Miriam Elisabet; Martínez Pastur, Guillermo José; Benavides, Maria Patricia; Curvetto, Nestor Raul; Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia; Royal Society of New Zealand; New Zealand Journal of Botany; 43; 2; 12-2005; 373-380
dc.identifier.issn
0028-825X
dc.identifier.uri
http://hdl.handle.net/11336/55768
dc.description.abstract
Although Berberis buxifolia has been reported to propagate in vitro, rooting is difficult to achieve during subcultures, with reduced quantity and quality of roots, as is the case with many other woody species. Several cofactors of rhizogenesis have been proposed, and the role of polyamines in rooting has recently acquired relevance. Polyamines can stimulate or inhibit microshoot rooting depending on the type and concentration of polyamine and rooting phase, while polyamine inhibitors can improve rooting in some cases. A study of the in vitro rooting of B. buxifolia using polyamines and a mix of polyamine inhibitors in a two-step culture medium is described here, in which a new successive rooting medium was successfully implemented. While polyamine inhibitors sometimes improved rooting, nutrient medium containing a low polyamine concentration (i.e., 1 μM) enhanced rooting compared with the control medium. The best micro-shoot rooting response resulted from the addition of 1 μM spermidine during the expression phase in the absence of inhibitors in both rooting phases. Overall, incorporating polyamine in the successive media enhances the quality and quantity of roots in B. buxifolia, thus confirming their role during the induction and expression of the radicle primordia differentiation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of New Zealand
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Berberis Buxifolia
dc.subject
Calafate
dc.subject
Micropropagation
dc.subject
Patagonia
dc.subject
Polyamine Inhibitors
dc.subject
Rhizogenesis
dc.subject
Root System Quality
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-11T14:18:25Z
dc.journal.volume
43
dc.journal.number
2
dc.journal.pagination
373-380
dc.journal.pais
Nueva Zelanda
dc.description.fil
Fil: Arena, Miriam Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Curvetto, Nestor Raul. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
New Zealand Journal of Botany
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/0028825X.2005.9512962
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/0028825X.2005.9512962


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