Artículo
Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1
Muglia, Cecilia Isabel
; Papa Gobbi, Rodrigo
; Smaldini, Paola Lorena
; Orsini Delgado, María Lucía
; Candia, Martín; Zanuzzi, Carolina Natalia
; Sambuelli, Alicia; Rocca, Andrés; Toscano, Marta Alicia
; Rabinovich, Gabriel Adrián
; Docena, Guillermo H.
Fecha de publicación:
07/2016
Editorial:
Wiley-liss, Div John Wiley & Sons Inc
Revista:
Journal of Cellular Physiology
ISSN:
0021-9541
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Galectins play key roles in the inflammatory cascade. In this study, we aimed to analyze the effect of galectin-1 (Gal-1) in the function of intestinal epithelial cells (IECs) isolated from healthy and inflamed mucosa. IECs isolated from mice or patients with inflammatory bowel diseases (IBD) were incubated with different pro-inflammatory cytokines, and Gal-1 binding, secretion of homeostatic factors and viability were assessed. Experimental models of food allergy and colitis were used to evaluate the in vivo influence of inflammation on Gal-1 binding and modulation of IECs. We found an enhanced binding of Gal-1 to: (a) murine IECs exposed to IL-1β, TNF, and IL-13; (b) IECs from inflamed areas in intestinal tissue from IBD patients; (c) small bowel of allergic mice; and (d) colon from mice with experimental colitis. Our results showed that low concentrations of Gal-1 favored a tolerogenic micro-environment, whereas high concentrations of this lectin modulated viability of IECs through mechanisms involving activation of caspase-9 and modulation of Bcl-2 protein family members. Our results showed that, when added in the presence of diverse pro-inflammatory cytokines such as tumor necrosis factor (TNF), IL-13 and IL-5, Gal-1 differentially promoted the secretion of growth factors including thymic stromal lymphopoietin (TSLP), epidermal growth factor (EGF), IL-10, IL-25, and transforming growth factor (TGF-β1). In conclusion, we found an augmented binding of Gal-1 to IECs when exposed in vitro or in vivo to inflammatory stimuli, showing different effects depending on Gal-1 concentration. These findings highlight the importance of the inflammatory micro-environment of mucosal tissues in modulating IECs susceptibility to the immunoregulatory lectin Gal-1 and its role in epithelial cell homeostasis.
Palabras clave:
Allergy
,
Colitis
,
Galectin-1
,
Gut
,
Inflammation
Archivos asociados
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Identificadores
Colecciones
Articulos(CIDCA)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos(IIFP)
Articulos de INST. DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Articulos de INST. DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Citación
Muglia, Cecilia Isabel; Papa Gobbi, Rodrigo; Smaldini, Paola Lorena; Orsini Delgado, María Lucía; Candia, Martín; et al.; Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Physiology; 231; 7; 7-2016; 1575-1585
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