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dc.contributor.author Raiden, Silvina Claudia
dc.contributor.author Schettini, Jorge
dc.contributor.author Salamone, Gabriela Veronica
dc.contributor.author Trevani, Analía Silvina
dc.contributor.author Vermeulen, Elba Monica
dc.contributor.author Gamberale, Romina
dc.contributor.author Giordano, Mirta Nilda
dc.contributor.author Geffner, Jorge Raúl
dc.date.available 2018-08-13T20:11:49Z
dc.date.issued 2003-04
dc.identifier.citation Raiden, Silvina Claudia; Schettini, Jorge; Salamone, Gabriela Veronica; Trevani, Analía Silvina; Vermeulen, Elba Monica; et al.; Human platelets produce granulocyte-macrophage colony-stimulating factor and delay eosinophil apoptosis; Nature Publishing Group; Laboratory Investigation; 83; 4; 4-2003; 589-598
dc.identifier.issn 0023-6837
dc.identifier.uri http://hdl.handle.net/11336/55221
dc.description.abstract An association between eosinophils and platelets has been described in several diseases, most notably asthma. Although the mechanisms through which platelets influence eosinophil behavior are not well defined, platelets seem to contribute to the selective accumulation of eosinophils at sites of allergic inflammation by virtue of their ability to produce eosinophil chemotactic factors. We report here for the first time that platelets delay apoptosis, thus enhancing eosinophil survival. A marked inhibition of spontaneous apoptosis was observed using eosinophil:platelet ratios of 1:50, 1:25, 1:10, and 1:5. Moreover, promotion of eosinophil apoptosis by either pronase or dexamethasone was also inhibited greatly in the presence of platelets. The antiapoptotic effect mediated by platelets was dependent on the release of soluble products and was significantly inhibited by neutralizing antibodies directed to GM-CSF. Studies performed by flow cytometry, directed to analyze the cellular source of this cytokine, demonstrated that intracytoplasmic GM-CSF is present in resting platelets. Moreover, GM-CSF was found in platelet supernatants, at concentrations able to prevent eosinophil apoptosis. Our findings support a novel mechanism through which platelets may contribute to eosinophil accumulation at allergic inflammatory sites.
dc.format application/pdf
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Human platelets produce granulocyte-macrophage colony-stimulating factor and delay eosinophil apoptosis
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-08-08T14:50:23Z
dc.journal.volume 83
dc.journal.number 4
dc.journal.pagination 589-598
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Raiden, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil Fil: Schettini, Jorge. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil Fil: Salamone, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil Fil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil Fil: Gamberale, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil Fil: Giordano, Mirta Nilda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil Fil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
dc.journal.title Laboratory Investigation
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1097/01.LAB.0000062851.71286.47
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/3780645
dc.conicet.fuente unificacion


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)