Determination of human sperm calcium uptake mediated by progesterone may be useful for evaluating unexplained sterility

Sperm samples from couples who underwent assisted reproduction were classified according to the World Health Organization (WHO) criteria of concentration, motility, and morphology, in normal and subnormal cases (oligozoospermic, asthenozoospermic, and teratozoospermic). The percentage of spermatozoa that increased [Ca 2+] i in response to progesterone (P) was determined by means of flow cytometry. The evaluation of the P-mediated intracellular calcium increase by flow cytometry may be a fast and objective tool for the diagnosis of human sperm samples, especially in cases of unexplained sterility. © 2004 by American Society for Reproductive Medicine.

Palabras clave: Calcium Uptake , Flow Cytometer , Human Spermatozoa , Progesterone

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

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URI: http://hdl.handle.net/11336/55157

URL: https://www.sciencedirect.com/science/article/pii/S0015028204009070

DOI: https://doi.org/10.1016/j.fertnstert.2003.12.051

Colecciones

Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA

Articulos(IIBYT)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS

Citación

Giojalas, Laura Cecilia; Iribarren, Pablo; Molina, Rosa Alejandra; Rovasio, Roberto Americo; Estofán, Daniel; Determination of human sperm calcium uptake mediated by progesterone may be useful for evaluating unexplained sterility; Elsevier Science Inc; Fertility and Sterility; 82; 3; 9-2004; 738-740

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