Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Soybean ubiquitous urease with purification facilitator: An addition to the moonlighting studies toolbox

Martinelli, Anne H.S.; Lopes, Fernanda C.; Broll, Valquiria; Defferrari, Marina S.; Ligabue-Braun, Rodrigo; Kappaun, Karine; Tichota, Deise M.; Fruttero, Leonardo LuisIcon ; Moyetta, Natalia RitaIcon ; Demartini, Diogo R.; Postal, Melissa; Medeiros-Silva, Monica; Becker-Ritt, Arlete Beatriz; Pasquali, Giancarlo; Carlini, Célia Regina R S
Fecha de publicación: 02/2017
Editorial: Elsevier
Revista: Process Biochemistry
ISSN: 1359-5113
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Ureases are nickel-dependent enzymes that catalyze the hydrolysis of urea to ammonia and carbon dioxide. In soybean (Glycine max), the embryo-specific urease (eSBU), the ubiquitous urease (uSBU), and a third isoform (SBU-III) are synthesized. Our group has previously demonstrated that eSBU, purified from seeds, has antifungal properties against phytopathogenic fungi, entomotoxicity against Dysdercus peruvianus, the ability to induce blood platelet aggregation, and these properties are independent of its enzymatic activity. Here we describe the biological properties of apo-uSBU fused to glutathione S-transferase (GST) produced in Escherichia coli. Removal of GST affected apo-uSBU stability. We performed a Response Surface Methodology to optimize GST-uSBU production to 5 mg per liter and then bioassays were carried out. The recombinant protein exhibited inhibitory effects on filamentous fungi and affected fungal secondary metabolism. Candida albicans and C. tropicalis were also susceptible to GST-uSBU and formed pseudo-hyphae. The fusion protein was toxic against Rhodnius prolixus, with the toxicity being accompanied by in vivo and in vitro hemocyte aggregation. Rabbit platelet also aggregated in the presence of GST-uSBU. Thus, uSBU displayed similar biological properties as previously described for eSBU even when fused to GST, reinforcing the proposed role of ureases in plant defense.
Palabras clave: Antifungal Activity , Fungal Pigments , Insecticidal Activity , Production Optimization , Soybean , Ubiquitous Urease
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 3.196Mb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/55116
URL: http://www.sciencedirect.com/science/article/pii/S1359511316310352
DOI: http://ri.conicet.gov.ar/admin/retrieve/fe161e5e-e98b-498a-83c0-114869bcb2a6/dx.
Colecciones
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Martinelli, Anne H.S.; Lopes, Fernanda C.; Broll, Valquiria; Defferrari, Marina S.; Ligabue-Braun, Rodrigo; et al.; Soybean ubiquitous urease with purification facilitator: An addition to the moonlighting studies toolbox; Elsevier; Process Biochemistry; 53; 2-2017; 245-258
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES