Artículo

Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells

Atzingen, Marina V.; Gomez, Ricardo MartinIcon ; Schattner, Mirta AnaIcon ; Prêtre, GabrielaIcon ; Goncalez, Amane P.; de Morais, Zenaide M.; Vasconcellos, Silvio A.; Nascimento. Ana L. T. O.
Fecha de publicación: 10/2009
Editorial: W B Saunders Co Ltd
Revista: The Journal Of Infection.
ISSN: 0163-4453
e-ISSN: 1532-2742
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

The study of a predicted outer membrane leptospiral protein encoded by the gene LIC12690 in mediating the adhesion process. The gene was cloned and expressed in Escherichia coli BL21 (SI) strain by using the expression vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and used to assess its ability to activate human umbilical vein endothelial cells (HUVECs).
Palabras clave: Endothelial Cells , Leptospira , Adhesion Molecules , E-Selectin , Gene Expression
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/55091
URL: https://www.journalofinfection.com/article/S0163-4453(09)00189-3/fulltext
DOI: http://dx.doi.org/10.1016/j.jinf.2009.07.010
Colecciones
Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
Atzingen, Marina V.; Gomez, Ricardo Martin; Schattner, Mirta Ana; Prêtre, Gabriela; Goncalez, Amane P.; et al.; Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells; W B Saunders Co Ltd; The Journal Of Infection.; 59; 4; 10-2009; 264-276
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