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Artículo

Down regulation of the proliferation and apoptotic pathways in the embryonic brain of diabetic rats

Kruse, Maria SolIcon ; Barutta, JoaquinIcon ; Vega, Maria CristinaIcon ; Coirini, HectorIcon
Fecha de publicación: 08/2012
Editorial: Springer/Plenum Publishers
Revista: Cellular And Molecular Neurobiology.
ISSN: 0272-4340
e-ISSN: 1573-6830
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Neurociencias

Resumen

Compelling evidence shows that the offspring subjected to uncontrolled hyperlycemia during gestation display behavioral, neurochemical, and cellular abnormalities during adulthood. However, the molecular mechanisms underlying these defects remain elusive. Previous studies have shown an increased rate of apoptosis and a decreased index of neuronal proliferation associated with diabetic embryopathy. The aim of the present study was to determine whether impairments in apoptotic related proteins also occur in the developing central nervous system from non-malformed embryos exposed to uncontrolled gestational hyperglycemia. Pregnant rats injected with either streptozotocin or vehicle were killed on gestational day 19. Offspring brains were quickly removed to evaluate protein expression by Western blotting. Embryonic brains from diabetic rats exhibited a decrease in the cell survival p-Akt expression (52.83 ± 24.35%) and in the pro-apoptotic protein Bax (56.16 ± 6.47%). Moreover, the antiapoptotic protein Bcl-2 showed a non-significant increase while there were no changes in Procaspase 3 or cleaved Caspase 3 proteins. The cytoskeleton proteins NF-200 and GFAP were also examined. Neither NF-200 nor GFAP showed differences in embryonic brains from diabetic rats compared to controls. Altogether, these results indicate that both proliferation and apoptotic pathways are decreased in the brain from the developing offspring of diabetic rats. Since selective neuronal apoptosis, as well as selective cell proliferation, are specifically involved in brain organogenesis, it is possible that simultaneous impairments during the perinatal period contribute to the long lasting alterations observed in the adult brain.
Palabras clave: Akt , Bax , Bcl-2 , Caspase 3 , Nf-200 , Brain Enzimology , Diabetes Mellitus
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/55086
URL: https://link.springer.com/article/10.1007/s10571-012-9820-8
DOI: http://dx.doi.org/10.1007/s10571-012-9820-8
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Kruse, Maria Sol; Barutta, Joaquin; Vega, Maria Cristina; Coirini, Hector; Down regulation of the proliferation and apoptotic pathways in the embryonic brain of diabetic rats; Springer/Plenum Publishers; Cellular And Molecular Neurobiology.; 32; 6; 8-2012; 1031-1037
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