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dc.contributor.author
Benavidez, Tomás Enrique
dc.contributor.author
Wechsler, Marissa E.
dc.contributor.author
Farrer, Madeleine M.
dc.contributor.author
Bizios, Rena
dc.contributor.author
Garcia, Carlos D.
dc.date.available
2018-08-10T17:42:19Z
dc.date.issued
2016-01
dc.identifier.citation
Benavidez, Tomás Enrique; Wechsler, Marissa E.; Farrer, Madeleine M.; Bizios, Rena; Garcia, Carlos D.; Electrochemically Preadsorbed Collagen Promotes Adult Human Mesenchymal Stem Cell Adhesion; Mary Ann Liebert; Tissue Engineering Part C-methods; 22; 1; 1-2016; 69-75
dc.identifier.issn
1937-3384
dc.identifier.uri
http://hdl.handle.net/11336/54968
dc.description.abstract
The present article reports on the effect of electric potential on the adsorption of collagen type I (the most abundant component of the organic phase of bone) onto optically transparent carbon electrodes (OTCE) and its mediation on subsequent adhesion of adult, human, mesenchymal stem cells (hMSCs). For this purpose, adsorption of collagen type I was investigated as a function of the protein concentration (0.01, 0.1, and 0.25 mg/mL) and applied potential (open circuit potential [OCP; control], +400, +800, and +1500 mV). The resulting substrate surfaces were characterized using spectroscopic ellipsometry, atomic force microscopy, and cyclic voltammetry. Adsorption of collagen type I onto OTCE was affected by the potential applied to the sorbent surface and the concentration of protein. The higher the applied potential and protein concentration, the higher the adsorbed amount (Γcollagen). It was also observed that the application of potential values higher than +800 mV resulted in the oxidation of the adsorbed protein. Subsequent adhesion of hMSCs on the OTCEs (precoated with the collagen type I films) under standard cell culture conditions for 2 h was affected by the extent of collagen preadsorbed onto the OTCE substrates. Specifically, enhanced hMSCs adhesion was observed when the Γcollagen was the highest. When the collagen type I was oxidized (under applied potential equal to +1500 mV), however, hMSCs adhesion was decreased. These results provide the first correlation between the effects of electric potential on protein adsorption and subsequent modulation of anchorage-dependent cell adhesion.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Mary Ann Liebert
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Biocampatibility
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Cell Adhesion
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Collagen Type I
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Human Mesenchymal Stem Cell
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Optically Transparent Carbon Films
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Protein Adsorption Assited by Potential
dc.subject.classification
Otras Biotecnologías de la Salud
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Biotecnología de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Electrochemically Preadsorbed Collagen Promotes Adult Human Mesenchymal Stem Cell Adhesion
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-30T15:24:31Z
dc.journal.volume
22
dc.journal.number
1
dc.journal.pagination
69-75
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Clemson University; Estados Unidos
dc.description.fil
Fil: Wechsler, Marissa E.. University of Texas; Estados Unidos
dc.description.fil
Fil: Farrer, Madeleine M.. University of Texas; Estados Unidos
dc.description.fil
Fil: Bizios, Rena. University of Texas; Estados Unidos
dc.description.fil
Fil: Garcia, Carlos D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Clemson University; Estados Unidos
dc.journal.title
Tissue Engineering Part C-methods
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/ten.tec.2015.0315
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1089/ten.TEC.2015.0315
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