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Artículo

Dietary iodide controls its own absorption through post-transcriptional regulation of the intestinal Na+/I- symporter

Nicola, Juan PabloIcon ; Reyna-Neyra, Andrea; Carrasco, Nancy; Masini, Ana María
Fecha de publicación: 12/2012
Editorial: Wiley Blackwell Publishing, Inc
Revista: The Journal Of Physiology
ISSN: 0022-3751
e-ISSN: 1469-7793
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Dietary I- absorption in the gastrointestinal tract is the first step in I- metabolism. Given that I- is an essential constituent of the thyroid hormones, its concentrating mechanism is of significant physiological importance. We recently described the expression of the Na+/I- symporter (NIS) on the apical surface of the intestinal epithelium as a central component of the I- absorption system and reported reduced intestinal NIS expression in response to an I--rich diet in vivo. Here, we evaluated the mechanism involved in the regulation of NIS expression by I- itself in enterocytes. Excess I- reduced NIS-mediated I- uptake in IEC-6 cells in a dose- and time-dependent fashion, which was correlated with a reduction of NIS expression at the plasma membrane. Perchlorate, a competitive inhibitor of NIS, prevented these effects, indicating that an increase in intracellular I- regulates NIS. Iodide induced rapid intracellular recruitment of plasma membrane NIS molecules and NIS protein degradation. Lower NIS mRNA levels were detected in response to I- treatment, although no transcriptional effect was observed. Interestingly, I- decreased NIS mRNA stability, affecting NIS translation. Heterologous green fluorescent protein-based reporter constructs revealed a significant repressive effect of the I--targeting NIS mRNA 3′ untranslated region. In conclusion, excess I- downregulates NIS expression in enterocytes by virtue of a complex mechanism. Our data suggest that I- regulates intestinal NIS mRNA expression at the post-transcriptional level as part of an autoregulatory effect of I- on its own metabolism. © 2012 The Authors. The Journal of Physiology © 2012 The Physiological Society.
Palabras clave: Na/I Symporter , Small Intestine , Iodide Absoption , Postranscriptional Regulation
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/54953
URL: https://physoc.onlinelibrary.wiley.com/doi/abs/10.1113/jphysiol.2012.241307
DOI: http://dx.doi.org/10.1113/jphysiol.2012.241307
Colecciones
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Nicola, Juan Pablo; Reyna-Neyra, Andrea; Carrasco, Nancy; Masini, Ana María; Dietary iodide controls its own absorption through post-transcriptional regulation of the intestinal Na+/I- symporter; Wiley Blackwell Publishing, Inc; The Journal Of Physiology; 590; 23; 12-2012; 6013-6026
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