Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Downregulation of Mac-1 Expression in Monocytes by Surface-Bound IgG

Fernández Calotti, Paula X.; Salamone, Gabriela VeronicaIcon ; Gamberale, RominaIcon ; Trevani, Analía SilvinaIcon ; Vermeulen, Elba MonicaIcon ; Geffner, Jorge RaúlIcon ; Giordano, Mirta NildaIcon
Fecha de publicación: 01/2003
Editorial: Wiley Blackwell Publishing, Inc
Revista: Scandinavian Journal Of Immunology
ISSN: 0300-9475
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Inmunología

Resumen

Physical and functional association between the β2-integrin Mac-1 (CD11b/ CD18) and receptors of immunoglobulin G (IgG) (FcγRs) has been previously reported. In this study, we examined the modulation of Mac-1 expression by IgG in different leucocyte populations. Our data show that human monocytes, but not neutrophils, macrophages, dendritic or natural killer cells, downregulate the expression of Mac-1 after overnight exposure to surface-bound IgG. This effect, which requires at least 6 h of incubation, is not associated with a general downmodulation of membrane antigens, and is selectively induced by immobilized IgG (iIgG), as the stimulation of monocytes with N-formyl-methionyl-leucyl-phenylalanine, lipopolysaccharide, tumour necrosis factor-α (TNF-α) or soluble IgG did not modify the Mac-1 expression after 18 h in culture. The loss of Mac-1 was completely prevented by blocking antibodies (Abs) directed to FcγRII (CD32) or CD 18. On the other hand, the serine protease inhibitor, phenyl methyl sulphonyl fluoride, but not inhibitors of cysteine proteases or neutral endopeptidases, partially prevented the downregulation of Mac-1 by iIgG. Monocytes cultured overnight on iIgG exhibited a dramatic decrease in their capacity to ingest zymosan particles that could be attributed to the reduced expression of Mac-1. However, there was no inhibition of TNF-α production induced by zymosan, suggesting that Mac-1-dependent responses require different levels of its expression to be fully activated.
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 359.4Kb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/54949
DOI: https://dx.doi.org/10.1046/j.1365-3083.2003.01187.x
URL: https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-3083.2003.01187.x
Colecciones
Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
Fernández Calotti, Paula X.; Salamone, Gabriela Veronica; Gamberale, Romina; Trevani, Analía Silvina; Vermeulen, Elba Monica; et al.; Downregulation of Mac-1 Expression in Monocytes by Surface-Bound IgG; Wiley Blackwell Publishing, Inc; Scandinavian Journal Of Immunology; 57; 1; 1-2003; 35-44
Compartir
Altmétricas
 
Estadísticas
Visualizaciones: 117
Descargas: 181

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • Twitter
  • Instagram
  • YouTube
  • Sound Cloud

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

Ministerio
https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES