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Artículo

Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone

Di Yorio, María PaulaIcon ; Pérez Sirkin, Daniela IrinaIcon ; Delgadin, Tomás HoracioIcon ; Shimizu, A.; Tsutsui, K.; Somoza, Gustavo ManuelIcon ; Vissio, Paula GabrielaIcon
Fecha de publicación: 05/2016
Editorial: Wiley Blackwell Publishing, Inc
Revista: Journal of Neuroendocrinology
ISSN: 0953-8194
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

The role of gonadotrophin-inhibitory hormone (GnIH) in the inhibition of the reproductive axis has been well-established in birds and mammals. However, its role in other vertebrates, such as the teleost fish, remains controversial. In this context, the present study aimed to evaluate whether GnIH modulates the release of gonadotrophins and growth hormone (GH) in the cichlid fish Cichlasoma dimerus. First, we partially sequenced the precursor polypeptide for GnIH and identified three putative GnIH peptides. Next, we analysed the expression of this precursor polypeptide via a polymerase chain reaction in the reproductive axis of both sexes. We found a high expression of the polypeptide in the hypothalamus and gonads of males. Immunocytochemistry allowed the observation of GnIH-immunoreactive somata in the nucleus posterioris periventricularis and the nucleus olfacto-retinalis, with no differences between the sexes. GnIH-immunoreactive fibres were present in all brain regions, with a high density in the nucleus lateralis tuberis and at both sides of the third ventricle. Finally, we performed in vitro studies on intact pituitary cultures to evaluate the effect of two doses (10−6 m and 10−8 m) of synthetic C. dimerus (cd-) LPQRFa-1 and LPQRFa-2 on the release of gonadotrophins and GH. We observed that cd-LPQRFa-1 decreased β-luteinising hormone (LH) and β-follicle-stimulating hormone (FSH) and also increased GH release to the culture medium. The release of β-FSH was increased only when it was stimulated with the higher cd-LPQRFa-2 dose. The results of the present study indicate that cd-LPQRFa-1, the cichlid fish GnIH, inhibits β-LH and β-FSH release and stimulates GH release in intact pituitary cultures of C. dimerus. The results also show that cd-LPQRF-2 could act as an β-FSH-releasing factor in this fish species.
Palabras clave: Fish , Follicle-Stimulating Hormone , Gonadotrophin-Inhibitory Hormone , Growth Hormone , Luteinising Hormone
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/54876
DOI: https://dx.doi.org/10.1111/jne.12377
URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/jne.12377
Colecciones
Articulos(IIB-INTECH) [759]
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(CCT - LA PLATA) [7034]
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IBBEA) [513]
Articulos de INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Citación
Di Yorio, María Paula; Pérez Sirkin, Daniela Irina; Delgadin, Tomás Horacio; Shimizu, A.; Tsutsui, K.; et al.; Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone; Wiley Blackwell Publishing, Inc; Journal of Neuroendocrinology; 28; 5; 5-2016
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