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dc.contributor.author
Zarate, Gabriela del Valle  
dc.contributor.author
Perez Chaia, Adriana Beatriz  
dc.date.available
2018-08-09T19:26:16Z  
dc.date.issued
2009-03  
dc.identifier.citation
Zarate, Gabriela del Valle; Perez Chaia, Adriana Beatriz; Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 106; 3; 3-2009; 1050-1057  
dc.identifier.issn
1364-5072  
dc.identifier.uri
http://hdl.handle.net/11336/54836  
dc.description.abstract
Aims: To assess in vitro the ability of some dairy bacteria to bind concanavalin A (Con A), peanut agglutinin (PNA) and jacalin (AIL), preventing their toxicity on mouse intestinal epithelial cells (IEC). Methods and Results: Con A and AIL reduced significantly IEC viability in vitro, as determined by Trypan Blue dye exclusion or by propidium iodide/fluorescein diacetate/Hoescht staining. Different strains of dairy bacteria were able to remove lectins from the media. Two strains were subjected to treatments used to remove S-layer, cell wall proteins, polysaccharides and lectin-like adhesins. They were then assayed for the ability to bind dietary lectins and reduce toxicity against IEC and to adhere to IEC after interaction with lectins. Con A and AIL were removed by Propionibacterium acidipropionici and Propionibacterium freudenreichii by binding with specific sugar moieties on the bacterial surface. Removal of lectins by bacteria impaired IEC protection. Adhesion of P. acidipropionici to IEC was reduced but not abolished after binding Con A or AIL. Conclusions: Removal of Con A or AIL by dairy propionibacteria was effective to avoid the toxic effect against colonic cells in vitro. Significance and Impact of the Study: Consumption of foods containing these bacteria would be a tool to protect the intestinal epithelia.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley Blackwell Publishing, Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Adhesion  
dc.subject
Colonocytes  
dc.subject
Cytotoxicity  
dc.subject
Lectins  
dc.subject
Propionibacteria  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-07-11T15:25:00Z  
dc.identifier.eissn
1365-2672  
dc.journal.volume
106  
dc.journal.number
3  
dc.journal.pagination
1050-1057  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina  
dc.description.fil
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina  
dc.journal.title
Journal of Applied Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1365-2672.2008.04077.x  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2672.2008.04077.x