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Artículo

Effect of thrombopoietin and granulocyte colony-stimulating factor on platelets and polymorphonuclear leukocytes

Schattner, Mirta AnaIcon ; Pozner, Roberto GabrielIcon ; Gorostizaga, Alejandra BeatrizIcon ; Lazzari, María ÁngelaIcon
Fecha de publicación: 07/2000
Editorial: Pergamon-Elsevier Science Ltd
Revista: Thrombosis Research
ISSN: 0049-3848
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Medicina Critica y de Emergencia

Resumen

Thrombopoietin (TPO) and granulocyte colony-stimulating factor (G-CSF) may be administered together in aplastic patients. We evaluated the effect of both cytokines alone or combined on platelets and polymorphonuclear leukocytes (PMN) functional responses. TPO, G-CSF, or the combination of both cytokines, induced neither platelet nor PMN activation. TPO but not G-CSF synergized with threshold ADP concentrations to induce maximal aggregation and ATP release. The synergistic effect of TPO with ADP was not modified by the presence of G-CSF. Flow cytometry studies have shown that thrombin-induced loss of GPIb from platelet surface was significantly increased by pretreatment of platelets with TPO, G-CSF, or both cytokines. P-selectin expression induced by thrombin was augmented by TPO, but not by G-CSF. Coincubation of the cells with TPO and G-CSF did not modify the values obtained with TPO alone. Expression of CD11b on PMN surface was augmented by G-CSF or fMLP. G-CSF-treated PMN increased the effect of fMLP on CD11b expression. TPO did not modify either basal levels of CD11b or the increased expression induced by G-CSF or fMLP. Incubation of PMN with both cytokines showed no differences compared to G-CSF alone. Platelet-PMN aggregates induced by thrombin in whole blood were augmented by TPO. G-CSF alone neither synergized with thrombin nor changed the results observed with TPO. These data show that in vitro functional responses of platelets, or PMN induced by TPO or G-CSF alone, were neither further increased nor inhibited by treatment of the cells with both cytokines. (C) 2000 Elsevier Science Ltd.
Palabras clave: Granulocyte Colony-Stimulating Factor , Platelets , Polymorphonuclear Leukocytes , Thrombopoietin
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/54808
DOI: https://dx.doi.org/10.1016/S0049-3848(00)00238-3
URL: https://www.sciencedirect.com/science/article/pii/S0049384800002383
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Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
Schattner, Mirta Ana; Pozner, Roberto Gabriel; Gorostizaga, Alejandra Beatriz; Lazzari, María Ángela; Effect of thrombopoietin and granulocyte colony-stimulating factor on platelets and polymorphonuclear leukocytes; Pergamon-Elsevier Science Ltd; Thrombosis Research; 99; 2; 7-2000; 147-154
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