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dc.contributor.author
Burzaco, Jaione
dc.contributor.author
Conde, Manuel
dc.contributor.author
Parada, Luis Antonio
dc.contributor.author
Zugaza, José L.
dc.contributor.author
Dehaye, Jean-Paul
dc.contributor.author
Marino, Aída
dc.date.available
2015-05-26T15:32:40Z
dc.date.issued
2013-06-24
dc.identifier.citation
Burzaco, Jaione; Conde, Manuel; Parada, Luis Antonio; Zugaza, José L.; Dehaye, Jean-Paul; Marino, Aída; ATP Antagonizes Thrombin-Induced Signal Transduction through 12(S)-HETE and cAMP; Public Library Science; Plos One; 8; 24-6-2013; 67117-67118;
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/545
dc.description.abstract
In this study we have investigated the role of extracellular ATP on thrombin induced-platelet aggregation (TIPA) in washed
human platelets. ATP inhibited TIPA in a dose-dependent manner and this inhibition was abolished by apyrase but not by
adenosine deaminase (ADA) and it was reversed by extracellular magnesium. Antagonists of P2Y1 and P2Y12 receptors had
no effect on this inhibition suggesting that a P2X receptor controlled ATP-mediated TIPA inhibition. ATP also blocked
inositol phosphates (IP1, IP2, IP3) generation and [Ca2+]i mobilization induced by thrombin. Thrombin reduced cAMP levels
which were restored in the presence of ATP. SQ-22536, an adenylate cyclase (AC) inhibitor, partially reduced the inhibition exerted by ATP on TIPA. 12-lipoxygenase (12-LO) inhibitors, nordihidroguaretic acid (NDGA) and 15(S)-hydroxy-5,8,11,13- eicosatetraenoic acid (15(S)-HETE), strongly prevented ATP-mediated TIPA inhibition. Additionally, ATP inhibited the increase of 12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12(S)-HETE) induced by thrombin. Pretreatment with both SQ- 22536 and NDGA almost completely abolished ATP-mediated TIPA inhibition. Our results describe for the first time that ATP implicates both AC and 12-LO pathways in the inhibition of human platelets aggregation in response to agonists.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Trombin
dc.subject
Platelet Aggregation
dc.subject
Atp
dc.subject
Signalling
dc.subject.classification
Ciencias Naturales y Exactas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
Bioquímica y Biología Molecular (ídem 3.1.10)
dc.title
ATP Antagonizes Thrombin-Induced Signal Transduction through 12(S)-HETE and cAMP
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.pagination
67117-67118
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Burzaco, Jaione. UNIVERSIDAD DEL PAIS VASCO;
dc.description.fil
Fil: Conde, Manuel. UNIVERSIDAD DEL PAIS VASCO;
dc.description.fil
Fil: Parada, Luis Antonio. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Salta. Instituto de Patologia Experimental;
dc.description.fil
Fil: Zugaza, José L.. UNIVERSIDAD DEL PAIS VASCO;
dc.description.fil
Fil: Dehaye, Jean-Paul. UNIVERSIDAD DEL PAIS VASCO;
dc.description.fil
Fil: Marino, Aída. UNIVERSIDAD DEL PAIS VASCO;
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0067117
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